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Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei

Seiboth, B ; Hakola, S ; Mach, R L ; Suominen, P L ; Kubicek, C P

Journal of bacteriology, September 1997, Vol.179(17), pp.5318-20 [Periódico revisado por pares]

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  • Título:
    Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei
  • Autor: Seiboth, B ; Hakola, S ; Mach, R L ; Suominen, P L ; Kubicek, C P
  • Assuntos: Cellulase -- Genetics ; Cellulose -- Pharmacology ; Gene Expression Regulation, Fungal -- Physiology ; Trichoderma -- Genetics
  • É parte de: Journal of bacteriology, September 1997, Vol.179(17), pp.5318-20
  • Descrição: The relative contributions of four major cellulases of Trichoderma reesei (1,4-beta-D-glucan cellobiohydrolase I [CBH I], CBH II, endo-1,4-beta-D-glucanase I [EG I], and EG II) to the generation of the cellulase inducer from cellulose were studied with isogenic strains in which the corresponding genes (cbh1, cbh2, egl1, and egl2) had been deleted by insertion of the Aspergillus nidulans amdS marker gene. During growth on lactose (a soluble carbon source provoking cellulase gene expression), these strains showed no significant alterations in their ability to express the respective other cellulase genes, with the exception of the strain containing delta cbh1, which exhibited an increased steady-state level of cbh2 mRNA. On crystalline cellulose as the only carbon source, however, significant differences were apparent: strains in which cbh2 and egl2, respectively, had been deleted showed no expression of the other cellulase genes, whereas strains carrying the cbh1 or egl1 deletion showed these...
  • Idioma: Inglês

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