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A mouse/human chimeric anti-(ganglioside GD3) antibody with enhanced antitumor activities

Shitara, K ; Kuwana, Y ; Nakamura, K ; Tokutake, Y ; Ohta, S ; Miyaji, H ; Hasegawa, M ; Hanai, N

Cancer immunology, immunotherapy : CII, June 1993, Vol.36(6), pp.373-80 [Periódico revisado por pares]

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  • Título:
    A mouse/human chimeric anti-(ganglioside GD3) antibody with enhanced antitumor activities
  • Autor: Shitara, K ; Kuwana, Y ; Nakamura, K ; Tokutake, Y ; Ohta, S ; Miyaji, H ; Hasegawa, M ; Hanai, N
  • Assuntos: Antibody-Dependent Cell Cytotoxicity ; Antibodies, Monoclonal -- Therapeutic Use ; Antineoplastic Agents -- Immunology ; Gangliosides -- Immunology ; Glioma -- Therapy ; Recombinant Fusion Proteins -- Immunology
  • É parte de: Cancer immunology, immunotherapy : CII, June 1993, Vol.36(6), pp.373-80
  • Descrição: Ganglioside GD3, which is one of the major gangliosides expressed on the cell surface of human tumors of neuroectodermal origin has been focused on as a target molecule for passive immunotherapy. We have cloned the cDNA encoding the immunoglobulin light and heavy chains of an anti-GD3 monoclonal antibody KM641 (murine IgG3, kappa), and constructed the chimeric genes by linking the cDNA fragments of the murine light and heavy variable regions to cDNA fragments of the human kappa and gamma 1 constant regions, respectively. The transfer of these cDNA constructs into SP2/0 mouse myeloma cells resulted in the production of the chimeric antibody, designated KM871, that retained specific binding activity to GD3. Indirect immunofluorescence revealed the same staining pattern for chimeric KM871 and the mouse counterpart KM641 on GD3-expressing melanoma cells. When human serum and human peripheral blood mononuclear cells were used as effectors in complement-mediated cytotoxicity and antibody-dependent...
  • Idioma: Inglês

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