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Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei

Cao, Yanli ; Zheng, Fanglin ; Wang, Lei ; Zhao, Guolei ; Chen, Guanjun ; Zhang, Weixin ; Liu, Weifeng

Molecular microbiology, July 2017, Vol.105(1), pp.65-83 [Periódico revisado por pares]

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  • Título:
    Rce1, a novel transcriptional repressor, regulates cellulase gene expression by antagonizing the transactivator Xyr1 in Trichoderma reesei
  • Autor: Cao, Yanli ; Zheng, Fanglin ; Wang, Lei ; Zhao, Guolei ; Chen, Guanjun ; Zhang, Weixin ; Liu, Weifeng
  • Assuntos: Cellulase -- Genetics ; Trichoderma -- Genetics
  • É parte de: Molecular microbiology, July 2017, Vol.105(1), pp.65-83
  • Descrição: Cellulase gene expression in the model cellulolytic fungus Trichoderma reesei is supposed to be controlled by an intricate regulatory network involving multiple transcription factors. Here, we identified a novel transcriptional repressor of cellulase gene expression, Rce1. Disruption of the rce1 gene not only facilitated the induced expression of cellulase genes but also led to a significant delay in terminating the induction process. However, Rce1 did not participate in Cre1-mediated catabolite repression. Electrophoretic mobility shift (EMSA) and DNase I footprinting assays in combination with chromatin immunoprecipitation (ChIP) demonstrated that Rce1 could bind directly to a cbh1 (cellobiohydrolase 1-encoding) gene promoter region containing a cluster of Xyr1 binding sites. Furthermore, competitive binding assays revealed that Rce1 antagonized Xyr1 from binding to the cbh1 promoter. These results indicate that intricate interactions exist between a variety of transcription factors to...
  • Idioma: Inglês

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