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Nuclear factor-like factor 2-antioxidant response element signaling activation by tert-butylhydroquinone attenuates acute heat stress in bovine mammary epithelial cells

Jin, X L ; Wang, K ; Liu, L ; Liu, H Y ; Zhao, F-Q ; Liu, J X

Journal of dairy science, November 2016, Vol.99(11), pp.9094-9103 [Periódico revisado por pares]

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  • Título:
    Nuclear factor-like factor 2-antioxidant response element signaling activation by tert-butylhydroquinone attenuates acute heat stress in bovine mammary epithelial cells
  • Autor: Jin, X L ; Wang, K ; Liu, L ; Liu, H Y ; Zhao, F-Q ; Liu, J X
  • Assuntos: Antioxidation ; Bovine Mammary Epithelial Cell ; Heat Shock ; Tbhq ; Antioxidant Response Elements ; Nf-E2-Related Factor 2 -- Genetics
  • É parte de: Journal of dairy science, November 2016, Vol.99(11), pp.9094-9103
  • Descrição: Nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) is a transcription factor that binds to the antioxidant response element (ARE) in the upstream promoter region of many antioxidative genes. The Nrf2-ARE signaling plays a key role in the cellular antioxidant-defense system, but whether Nrf2 activation has protective effects against heat shock (HS) stress in mammary epithelial cells (MEC) remains unclear. The objective of this study was to determine whether tert-butylhydroquinone (tBHQ), a well-known Nrf2 activator, could attenuate heat stress-induced cell damage in MAC-T cells of the bovine MEC line. The MAC-T cells were exposed to HS (42.5°C for 1h) followed by recovery at 37°C to mimic HS. Compared with cells that were consistently cultured at normothermia (37°C), the cell viability levels significantly decreased after HS stress. In parallel, heat stress increased the reactive oxygen species levels and induced cellular apoptosis and endoplasmic reticulum stress. The MAC-T cells...
  • Idioma: Inglês

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