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Flippase (FLP) recombinase-mediated marker recycling in the dermatophyte Arthroderma vanbreuseghemii

Yamada, Yohko ; Maeda, Mari ; Alshahni, Mohamed Mahdi ; Monod, Michel ; Staib, Peter ; Yamada, Tsuyoshi

Microbiology (Reading, England), October 2014, Vol.160(Pt 10), pp.2122-2135 [Periódico revisado por pares]

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  • Título:
    Flippase (FLP) recombinase-mediated marker recycling in the dermatophyte Arthroderma vanbreuseghemii
  • Autor: Yamada, Yohko ; Maeda, Mari ; Alshahni, Mohamed Mahdi ; Monod, Michel ; Staib, Peter ; Yamada, Tsuyoshi
  • Assuntos: Genetic Markers ; Recombination, Genetic ; Arthrodermataceae -- Genetics ; DNA Nucleotidyltransferases -- Metabolism ; Gene Targeting -- Methods ; Genetics, Microbial -- Methods
  • É parte de: Microbiology (Reading, England), October 2014, Vol.160(Pt 10), pp.2122-2135
  • Descrição: Biological processes can be elucidated by investigating complex networks of relevant factors and genes. However, this is not possible in species for which dominant selectable markers for genetic studies are unavailable. To overcome the limitation in selectable markers for the dermatophyte Arthroderma vanbreuseghemii (anamorph: Trichophyton mentagrophytes), we adapted the flippase (FLP) recombinase-recombination target (FRT) site-specific recombination system from the yeast Saccharomyces cerevisiae as a selectable marker recycling system for this fungus. Taking into account practical applicability, we designed FLP/FRT modules carrying two FRT sequences as well as the flp gene adapted to the pathogenic yeast Candida albicans (caflp) or a synthetic codon-optimized flp (avflp) gene with neomycin resistance (nptII) cassette for one-step marker excision. Both flp genes were under control of the Trichophyton rubrum copper-repressible promoter (PCTR4). Molecular analyses of resultant transformants...
  • Idioma: Inglês

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