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Altered expression of hepatic metabolic enzyme and apoptosis-related gene transcripts in human hepatocytes treated with trichloroethylene

Xu, X Y ; Liu, Y F ; Lu, L W ; Ke, Y B ; Mao, J Y ; Mao, K L

Human & experimental toxicology, September 2012, Vol.31(9), pp.861-7 [Periódico revisado por pares]

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  • Título:
    Altered expression of hepatic metabolic enzyme and apoptosis-related gene transcripts in human hepatocytes treated with trichloroethylene
  • Autor: Xu, X Y ; Liu, Y F ; Lu, L W ; Ke, Y B ; Mao, J Y ; Mao, K L
  • Assuntos: Gene Expression Regulation -- Drug Effects ; Hepatocytes -- Drug Effects ; Solvents -- Toxicity ; Trichloroethylene -- Toxicity
  • É parte de: Human & experimental toxicology, September 2012, Vol.31(9), pp.861-7
  • Descrição: Trichloroethylene (TCE) is a common organic solvent that has been widely used in industrial applications. Hundred cases of allergic reactions occurred after the workers were occupationally exposed to TCE in China in the past decade, but the underlying effector mechanisms of TCE remain unclear. The purpose of the present study is to examine the alteration of hepatic metabolic enzyme gene and apoptosis-related gene messenger RNA (mRNA) expression in L02 human hepatocytes (L02 cells) after treatment with TCE. L02 cells were cultured either with various doses of TCE (0.25, 0.5, 1.0 and 2.0 mmol/L) for 24 h or with a single dose of TCE (1.0 mmol/L) for different time intervals, whereas samples treated with dimethyl sulfoxide served as control. Quantitative real-time polymerase chain reaction analysis was performed to detect the mRNA expression of hepatic metabolic enzyme genes (CYP1A2, CYP3A4 and CYP2E1) and apoptosis-related genes (BAX and BAD). It was found that the transcript levels of hepatic...
  • Idioma: Inglês

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