skip to main content

Lacking expression of paternally-expressed gene confirms the failure of syngamy after intracytoplasmic sperm injection in swamp buffalo (Bubalus bubalis)

Chankitisakul, V ; Tharasanit, T ; Phutikanit, N ; Tasripoo, K ; Nagai, T ; Techakumphu, M

Theriogenology, 15 April 2012, Vol.77(7), pp.1415-24 [Periódico revisado por pares]

Texto completo disponível

Citações Citado por
  • Título:
    Lacking expression of paternally-expressed gene confirms the failure of syngamy after intracytoplasmic sperm injection in swamp buffalo (Bubalus bubalis)
  • Autor: Chankitisakul, V ; Tharasanit, T ; Phutikanit, N ; Tasripoo, K ; Nagai, T ; Techakumphu, M
  • Assuntos: Sperm-Ovum Interactions ; Buffaloes -- Genetics ; Sperm Injections, Intracytoplasmic -- Veterinary
  • É parte de: Theriogenology, 15 April 2012, Vol.77(7), pp.1415-24
  • Descrição: The objectives were to: (1) examine the efficiency of intracytoplasmic sperm injection (ICSI) technique, with or without chemical activation of in vitro matured buffalo oocytes, on sperm head decondensation; and (2) compare the subsequent development of embryos after activation of ICSI (ICSI (+) activation group) and sham injection (Sham (+) activation group) oocytes (embryos obtained by in vitro fertilization of IVM oocytes served as a control group). Pronuclear formation rates in ICSI (+) activation and Sham (+) activation groups were higher than that of ICSI without activation (P < 0.05). However, because 90.9% of presumptive zygotes in ICSI (+) activation group demonstrated pronuclear formation with an intact sperm head, we inferred that most were parthenotes. Neither developmental competence (morula and blastocyst formation rates) nor mean total cell number of blastocysts was significantly different among ICSI (+) activation, Sham (+) activation, and IVF groups. To clarify whether...
  • Idioma: Inglês

Buscando em bases de dados remotas. Favor aguardar.