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Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry

Theel, Elitza S ; Hall, Leslie ; Mandrekar, Jayawant ; Wengenack, Nancy L

Journal of clinical microbiology, December 2011, Vol.49(12), pp.4067-71 [Periódico revisado por pares]

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  • Título:
    Dermatophyte identification using matrix-assisted laser desorption ionization-time of flight mass spectrometry
  • Autor: Theel, Elitza S ; Hall, Leslie ; Mandrekar, Jayawant ; Wengenack, Nancy L
  • Assuntos: Arthrodermataceae -- Chemistry ; Microbiological Techniques -- Methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization -- Methods
  • É parte de: Journal of clinical microbiology, December 2011, Vol.49(12), pp.4067-71
  • Descrição: The performance of the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometer (MS) for the identification of dermatophytes from clinical cultures was compared to that of dermatophyte identification using 28S rRNA gene sequencing. The MALDI Biotyper library (MBL; version 3.0) was used alone and in combination with a supplemented library containing an additional 20 dermatophyte spectra (S-MBL). Acquired spectra were interpreted using both the manufacturer-recommended scores (genus, ≥1.7; species, ≥2.0) and adjusted cutoff values established by this study (genus, ≥1.5; species, ≥1.7); identifications required a minimum 10% difference in scores between the top two different organisms to be considered correct. One hundred well-characterized, archived dermatophyte isolates and 71 fresh dermatophyte cultures were evaluated using both libraries and both sets of cutoff criteria. Collectively, the S-MBL significantly outperformed the MBL at both the...
  • Idioma: Inglês

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