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Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation

Masumoto, Chisato ; Miyazawa, Shin-Ichi ; Ohkawa, Hiroshi ; Fukuda, Takuya ; Taniguchi, Yojiro ; Murayama, Seiji ; Kusano, Miyako ; Saito, Kazuki ; Fukayama, Hiroshi ; Miyao, Mitsue

Proceedings of the National Academy of Sciences of the United States of America, 16 March 2010, Vol.107(11), pp.5226-31 [Periódico revisado por pares]

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  • Título:
    Phosphoenolpyruvate carboxylase intrinsically located in the chloroplast of rice plays a crucial role in ammonium assimilation
  • Autor: Masumoto, Chisato ; Miyazawa, Shin-Ichi ; Ohkawa, Hiroshi ; Fukuda, Takuya ; Taniguchi, Yojiro ; Murayama, Seiji ; Kusano, Miyako ; Saito, Kazuki ; Fukayama, Hiroshi ; Miyao, Mitsue
  • Assuntos: Chloroplasts -- Enzymology ; Oryza -- Enzymology ; Phosphoenolpyruvate Carboxylase -- Metabolism ; Quaternary Ammonium Compounds -- Metabolism
  • É parte de: Proceedings of the National Academy of Sciences of the United States of America, 16 March 2010, Vol.107(11), pp.5226-31
  • Descrição: Phosphoenolpyruvate carboxylase (PEPC) is a key enzyme of primary metabolism in bacteria, algae, and vascular plants, and is believed to be cytosolic. Here we show that rice (Oryza sativa L.) has a plant-type PEPC, Osppc4, that is targeted to the chloroplast. Osppc4 was expressed in all organs tested and showed high expression in the leaves. Its expression in the leaves was confined to mesophyll cells, and Osppc4 accounted for approximately one-third of total PEPC protein in the leaf blade. Recombinant Osppc4 was active in the PEPC reaction, showing V(max) comparable to cytosolic isozymes. Knockdown of Osppc4 expression by the RNAi technique resulted in stunting at the vegetative stage, which was much more marked when rice plants were grown with ammonium than with nitrate as the nitrogen source. Comparison of leaf metabolomes of ammonium-grown plants suggested that the knockdown suppressed ammonium assimilation and subsequent amino acid synthesis by reducing levels of organic acids, which...
  • Idioma: Inglês

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