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Molecular sex identification of juvenile skeletal remains from an Irish medieval population using ancient DNA analysis

Tierney, S.N. ; Bird, J.M.

Journal of Archaeological Science, 2015, Vol.62, p.27(12) [Periódico revisado por pares]

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  • Título:
    Molecular sex identification of juvenile skeletal remains from an Irish medieval population using ancient DNA analysis
  • Autor: Tierney, S.N. ; Bird, J.M.
  • Assuntos: Genetic Research – Analysis ; Archaeology – Analysis ; DNA – Analysis ; Excavations (Archaeology) – Analysis
  • É parte de: Journal of Archaeological Science, 2015, Vol.62, p.27(12)
  • Descrição: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.jas.2015.06.016 Byline: S.N. Tierney, J.M. Bird Abstract: The archaeological excavation of a medieval cemetery in North West Ireland led to the recovery of the largest collection of human remains from a burial ground in Ireland to date. This collection included a substantial number of juvenile remains. In order to enhance the interpretation of the assemblage and give a more complete picture of the population, a sample of the juvenile population from Ballyhanna were sexed using DNA based techniques so that the mortality ratio of the male and female non-adult individuals could be assessed. Sex identification of human remains is generally assigned using the morphology of the skeleton or on some occasions using associated grave goods. However in instances when an assemblage contains immature or fragmentary material, an alternative and reliable means of sexing these individuals is required. Ancient DNA research in recent years has proven itself to be such a reliable alternative. In this study the reliability and reproducibility of two PCR based sexing methods were evaluated first on 38 adults of known sex to determine the accuracy of these methods for sexing individuals from the Ballyhanna assemblage. Using real time PCR and STR profiling systems, a dependable and consistent sexing system was developed. The reproducibility of the amplified samples meant that the methods were valid and subsequently could be used to sex juveniles. The molecular sexing results from the juveniles sampled determined that four of these juvenile individuals were males, 10 were probable males, one was a probable female and four were inconclusive. The results from this study, although not fully representative of the juvenile population excavated, indicate an excess of male mortality. Author Affiliation: School of Science, Institute of Technology, Sligo, Ash Lane, Sligo, Ireland Article History: Received 1 April 2014; Revised 18 June 2015; Accepted 21 June 2015
  • Idioma: Inglês

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