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Increasing fat content from 20 to 45 wt% in a complex diet induces lower endotoxemia in parallel with an increased number of intestinal goblet cells in mice

Nutrition Research, 2015, Vol.35(4), p.346(11) [Periódico revisado por pares]

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  • Título:
    Increasing fat content from 20 to 45 wt% in a complex diet induces lower endotoxemia in parallel with an increased number of intestinal goblet cells in mice
  • Assuntos: Leptin – Physiological Aspects ; Leptin – Analysis ; Leptin – Statistics ; Lipids – Physiological Aspects ; Lipids – Analysis ; Lipids – Statistics ; Body Weight – Physiological Aspects ; Body Weight – Analysis ; Body Weight – Statistics
  • É parte de: Nutrition Research, 2015, Vol.35(4), p.346(11)
  • Descrição: To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.nutres.2015.01.005 Byline: Berengere Benoit, Fabienne Laugerette, Pascale Plaisancie, Alain Geloen, Jacques Bodennec, Monique Estienne, Gaelle Pineau, Annick Bernalier-Donadille, Hubert Vidal, Marie-Caroline Michalski Abstract: The impacts of high-fat diets (HFDs) on the onset of metabolic endotoxemia and low-grade inflammation are well established in rodent models. However, the dose-effect of dietary lipid intakes on these parameters is not known. We hypothesized that increasing dietary lipid amounts could be linked to parallel increases of endotoxemia, low-grade inflammation, and metabolic and intestinal alterations. Six-week-old male C57BL/6J mice were fed a low-fat diet (LFD, 2.6 wt% of lipids), a moderate HFD (mHFD, 22 wt% of lipids), or a very HFD (vHFD, 45 wt% of lipids) formulated mainly using chow ingredients and milk fat. After 12 weeks, white adipose tissues, liver, intestine, distal colon contents, and plasma were collected. Only vHFD mice significantly increased body weight and fat mass vs LFD mice. This was associated with increases of plasma concentrations of triglycerides, leptin and adiponectin, and liver lipids. No such differences were observed between LFD and mHFD mice. However, mHFD developed metabolic endotoxemia and inflammation, unlike vHFD mice. In turn, vHFD mice showed more goblet cells in all intestine segments vs both other groups and a decrease of Bacteroides-Prevotella in their microbiota vs LFD mice. Finally, mHFD mice colon exhibited a decrease in lactobacilli and in the levels of occludin phosphorylation. Altogether, using complex HFD, no associations were observed between dietary lipid amounts and the magnitude of endotoxemia, inflammation, and physiological alterations developed. These results reveal the impact of the diet composition on intestinal goblet cells and mucus coat, bringing new insights about further consequences on HFD-induced metabolic disorders. Article History: Received 28 August 2014; Revised 12 January 2015; Accepted 15 January 2015
  • Idioma: Inglês

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