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Effect of silybin on phorbol myristate acetate-induced protein kinase C translocation, NADPH oxidase activity and apoptosis in human neutrophils

Varga, Zs. ; Ujhelyi, L. ; Kiss, A. ; Balla, J. ; Czompa, A. ; Antus, S.

Phytomedicine: International Journal of Phytotherapy & Phytopharmacology, Feb, 2004, Vol.11(2-3), p.206(7) [Periódico revisado por pares]

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  • Título:
    Effect of silybin on phorbol myristate acetate-induced protein kinase C translocation, NADPH oxidase activity and apoptosis in human neutrophils
  • Autor: Varga, Zs. ; Ujhelyi, L. ; Kiss, A. ; Balla, J. ; Czompa, A. ; Antus, S.
  • Assuntos: Nicotinamide Adenine Dinucleotide -- Health Aspects ; Oxidative Stress -- Case Studies ; Protein Kinases -- Health Aspects
  • É parte de: Phytomedicine: International Journal of Phytotherapy & Phytopharmacology, Feb, 2004, Vol.11(2-3), p.206(7)
  • Descrição: Summary Mechanism of the action of silybin (1) and its derivatives (2–4), possessing different lipid solubility in PMA-stimulated neutrophils was evaluated. Silybin (1) inhibited the calcium, phosphatidylserine- and diacylglycerol-dependent protein kinase C translocation and the NADPH oxidase activity in PMA-stimulated neutrophils and resulted in decreased apoptosis. Furthermore, silybin (1) inhibited xanthine oxidase activity and hem-mediated oxidative degradation of low-density lipoprotein, as well. Its derivatives (2–4), possessing different lipid-solubility, affected all the studied parameters. The lipid solubility of silybin (1) was enhanced by methylation (5′7′4″trimethylsilybin: 2), whereas a decrease in lipid-solubility by acetylation of compound 2 (5′,7,′4″-trimethylsilybin-acetate: 3) or all the hydroxyl groups of silybin (peracetyl-silybin: 4) attenuated the antioxidant capacity by decreasing the inhibition in PKC translocation and NADPH oxidase activation. All the derivatives of silybin (2–4) showed no inhibition in cell free systems; e.g. did not alter the xanthine oxidase activity and the hem-mediated oxidative degradation of LDL. In conclusion, the antioxidant activity of (1) might be due to its ability to inhibit PKC translocation and NADPH oxidase activation in PMA-stimulated neutrophils. The increase of lipid solubility of silybin (1) supports its penetration through cell membrane and enhances its inhibitory effects. This structural modification of (1) might have pharmacological consequences.
  • Idioma: English

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