skip to main content

Improving α, ω-dodecanedioic acid productivity from n-dodecane and hydrolysate of Candida cells by membrane integrated repeated batch fermentation

Cao, Weifeng ; Wang, Yujue ; Luo, Jianquan ; Yin, Junxiang ; Wan, Yinhua

Bioresource Technology, July 2018, Vol.260, pp.9-15 [Periódico revisado por pares]

Texto completo disponível

Citações Citado por
  • Título:
    Improving α, ω-dodecanedioic acid productivity from n-dodecane and hydrolysate of Candida cells by membrane integrated repeated batch fermentation
  • Autor: Cao, Weifeng ; Wang, Yujue ; Luo, Jianquan ; Yin, Junxiang ; Wan, Yinhua
  • Assuntos: Α, Ω-Dodecanedioic Acid ; Hydrolysate of Candida Cells ; Candida Viswanathii ; Α, Ω-Dicarboxylic Acids ; Membrane Bioreactor ; Agriculture ; Engineering ; Chemistry
  • É parte de: Bioresource Technology, July 2018, Vol.260, pp.9-15
  • Descrição: Keywords [alpha], [omega]-Dodecanedioic acid; Hydrolysate of Candida cells; Candida viswanathii; [alpha], [omega]-Dicarboxylic acids; Membrane bioreactor Highlights * Cells and n-dodecane were simultaneously recycled by membrane technology. * Hydrolysate of Candida cells was successfully used as a nitrogen source to replace 50% yeast extract. * [alpha], [omega]-Dodecanedioic acid productivity was highly enhanced with a membrane bioreactor. * This process is green, economical and effective. Abstract The aim of the present study is to develop an effective production process for [alpha], [omega]-dodecanedioic acid (DC.sub.12) biosynthesis using n-dodecane and hydrolysate of Candida cells as substrates by membrane integrated repeated batch fermentation. Cells and n-dodecane were simultaneously recycled during the filtration of fermentation broth (FB) with a 150 kDa ceramic membrane under a cross-flow velocity of 4 m/s and a trans-membrane pressure of 0.2 MPa, and it was also revealed that the cells in the broth could alleviate the membrane fouling during the FB filtration. Moreover, the hydrolysate of the collected cells could be successfully used as a nitrogen source to replace 50% yeast extract for decreasing the DC.sub.12 production cost. With repeated-batch culture in a membrane bioreactor, the maximal DC.sub.12 productivity could be enhanced by 57.8% compared with the batch culture, meanwhile n-dodecane and cells could be recovered and used for the next fermentation cycle. Author Affiliation: (a) State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China (b) University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, China (c) China National Center for Biotechnology Development, Beijing 100036, PR China * Corresponding author at: State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China. Article History: Received 24 January 2018; Revised 12 March 2018; Accepted 13 March 2018 Byline: Weifeng Cao (a), Yujue Wang (a,b), Jianquan Luo (a,b), Junxiang Yin (c), Yinhua Wan [yhwan@ipe.ac.cn] (a,b,*)
  • Idioma: Inglês

Buscando em bases de dados remotas. Favor aguardar.