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DETERMINATION OF PHYSICOCHEMICAL, IMMUNOCHEMICAL AND ANTIOXIDANT PROPERTIES, TOXICOLOGICAL AND HYGIENIC ASSESSMENT OF WHEY PROTEIN COMCENTRATE AND ITS HYDROLYSATE

Halavach T.M ; Kurchenko V.P ; Zhygankov V.G ; Evdokimov I.A

Foods and Raw Materials, 01 November 2015, Vol.3(2), pp.105-114 [Periódico revisado por pares]

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  • Título:
    DETERMINATION OF PHYSICOCHEMICAL, IMMUNOCHEMICAL AND ANTIOXIDANT PROPERTIES, TOXICOLOGICAL AND HYGIENIC ASSESSMENT OF WHEY PROTEIN COMCENTRATE AND ITS HYDROLYSATE
  • Autor: Halavach T.M ; Kurchenko V.P ; Zhygankov V.G ; Evdokimov I.A
  • Assuntos: Allergenic Milk Proteins ; Protein Hydrolysates ; Peptide Composition ; Residual Antigenicity ; Antioxidant Properties ; Toxicological and Hygienic Assessment ; Infusoria Tetrahymena Pyriformis
  • É parte de: Foods and Raw Materials, 01 November 2015, Vol.3(2), pp.105-114
  • Descrição: Enzymatic hydrolysis of whey proteins is aimed to obtain products with low allergenic potential and high nutritional value. Whey peptides are protein fraction that possesses a variety of physicochemical, immunochemical and bioactive properties (antioxidant, antibacterial, immunomodulatory effects). Controlled parameters of enzymatic hydrolysates are the degree of hydrolysis of protein substrates, peptide composition, residual antigenicity, antioxidant capacity, etc. The purpose of this work is to characterize peptide profile, antigenicity and free radical scavenging properties of experimental hydrolysate sample, toxicological and hygienic assessment of its impact on a test object (infusoria Tetrahymena pyriformis). The research of properties of whey proteins and their enzymatic hydrolysates was conducted using classical and modern methodological approaches: SDS electrophoresis in polyacrylamide gel, HPLC, mass-spectrometry, competitive ELISA, TEAC (Trolox Equivalent Capacity Assay)...
  • Idioma: Inglês

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