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General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.

Duncan, Caia Deborah ; Rodríguez-López, María ; Ruis, Phil ; Bähler, Jürg ; Mata, Juan Ignacio Apollo - University of Cambridge Repository; Apollo - University of Cambridge Repository

Apollo - University of Cambridge Repository 2018

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  • Título:
    General amino acid control in fission yeast is regulated by a nonconserved transcription factor, with functions analogous to Gcn4/Atf4.
  • Autor: Duncan, Caia Deborah ; Rodríguez-López, María ; Ruis, Phil ; Bähler, Jürg ; Mata, Juan Ignacio
  • Apollo - University of Cambridge Repository; Apollo - University of Cambridge Repository
  • Assuntos: Schizosaccharomyces ; Amino Acids ; Fungal Proteins ; Transcription Factors ; Gene Expression Regulation, Fungal
  • Descrição: Eukaryotes respond to amino acid starvation by enhancing the translation of mRNAs encoding b-ZIP family transcription factors (GCN4 in Saccharomyces cerevisiae and ATF4 in mammals), which launch transcriptional programs to counter this stress. This pathway involves phosphorylation of the eIF2 translation factor by Gcn2-protein kinases, and is regulated by uORFs in the GCN4/ATF4 5’-leaders. Here we present evidence that the transcription factors that mediate this response are not evolutionary conserved. Although cells of the fission yeast Schizosaccharomyces pombe respond transcriptionally to amino acid starvation, they lack clear Gcn4 and Atf4 orthologs. We used ribosome profiling to identify mediators of this response in S. pombe, looking for transcription factor that behave like GCN4. We discovered a novel transcription factor (Fil1) translationally induced by amino acid starvation in a 5’-leader and Gcn2-dependent manner. Like Gcn4, Fil1 is required for the transcriptional response to amino acid starvation, and Gcn4 and Fil1 regulate similar genes. Despite their similarities in regulation, function, and targets, Fil1 and Gcn4 belong to different transcription factors families (GATA and b-ZIP, respectively). Thus, the same functions are performed by non-orthologous proteins under similar regulation. These results highlight the plasticity of transcriptional networks, which maintain conserved principles with non-conserved regulators....
  • Editor: Apollo - University of Cambridge Repository
  • Data de publicação: 2018

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