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Inhibition of the glucose and amino‐acid carriers of Lemna gibba by pretreatment with HgCl 2

Golle, Bernd ; Lüttge, Ulrich

Physiologia Plantarum, January 1983, Vol.57(1), pp.62-66 [Periódico revisado por pares]

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  • Título:
    Inhibition of the glucose and amino‐acid carriers of Lemna gibba by pretreatment with HgCl 2
  • Autor: Golle, Bernd ; Lüttge, Ulrich
  • Assuntos: Ecotoxicily ; Proton‐Cotransport ; Sh‐Groups
  • É parte de: Physiologia Plantarum, January 1983, Vol.57(1), pp.62-66
  • Descrição: The electrical resting potential across the plasmalemma of L. (G 1) cells is −230 to −250 mV and the diffusion potential in the presence of 1 mol m KCN + 1 mol m salicylhydroxamic acid is about −100 mV. A concentration of 0.01 mol m HgCl depolarises the transmembrane electrical potential in a largely reversible way. When the cells after 16 min of HgCl‐application are returned to Hg‐free solution, the transmembrane electrical potential is only depolarised by 24 × 13 mV (SD, n = 13) compared with the potential prior to HgCl treatment. In contrast, a 16 min pretreatment with HgCl followed by a wash with mercury‐free solution reduces the transient depolarisations of transmembrane potential observed after addition of 5 mol m D‐glncose or 1 mol m L‐alaoine to about 60% of controls. These transient depolarisations are due to the onset of solute uptake. Accordingly, HgCl‐pretreatment inhibits uptake of C‐3‐O‐methyl‐‐glucose by more than 50% and uptake of C‐‐alanine by more than 70%. Washing with 1 mol m 1,4‐dithiothreitol does not reverse this inhibition. It is, therefore, concluded that Hg irreversibly binds to essential SH‐groups of the H‐hexose and the H‐amino‐acid cotransport carriers of and inhibits these carriers without appreciably affecting the electrogenic proton‐extrusion pump.

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