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DNA in ancient bone – Where is it located and how should we extract it?

Campos, Paula F ; Craig, Oliver E ; Turner-Walker, Gordon ; Peacock, Elizabeth ; Willerslev, Eske ; Gilbert, M. Thomas P

Annals of Anatomy, 2012, Vol.194(1), pp.7-16 [Periódico revisado por pares]

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  • Título:
    DNA in ancient bone – Where is it located and how should we extract it?
  • Autor: Campos, Paula F ; Craig, Oliver E ; Turner-Walker, Gordon ; Peacock, Elizabeth ; Willerslev, Eske ; Gilbert, M. Thomas P
  • Assuntos: Bone ; Ancient DNA ; DNA Extraction ; Collagen ; Hydroxyapatite ; Zoology
  • É parte de: Annals of Anatomy, 2012, Vol.194(1), pp.7-16
  • Descrição: Despite the widespread use of bones in ancient DNA (aDNA) studies, relatively little concrete information exists in regard to how the DNA in mineralised collagen degrades, or where it survives in the material's architecture. While, at the macrostructural level, physical exclusion of microbes and other external contaminants may be an important feature, and, at the ultrastructural level, the adsorption of DNA to hydroxyapatite and/or binding of DNA to Type I collagen may stabilise the DNA, the relative contribution of each, and what other factors may be relevant, are unclear. There is considerable variation in the quality of DNA retrieved from bones and teeth. This is in part due to various environmental factors such as temperature, proximity to free water or oxygen, pH, salt content, and exposure to radiation, all of which increase the rate of DNA decay. For example, bone specimens from sites at high latitudes usually yield better quality DNA than samples from temperate regions,...
  • Idioma: Inglês

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