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Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line

Martha Silveira e Costa Marcelo Faulhaber; Maria Cristina Marcucci; Martin Andreas Metzger; Sergio Paulo Bydlowski; Ana Carolina Oliveira; Estela Maria Novak; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)

Abstracts Campinas, SP

Campinas 2004

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  • Título:
    Inhibitory effect of phenolic acid derivatives from Brazilian propolis on cell growth in a human multiple myeloma cell line
  • Autor: Martha Silveira e Costa
  • Marcelo Faulhaber; Maria Cristina Marcucci; Martin Andreas Metzger; Sergio Paulo Bydlowski; Ana Carolina Oliveira; Estela Maria Novak; Congress of the Brazilian Society for Cell Biology (12. 2004 Campinas); Ibero-American Congress of Cell Biology (9. 2004 Campinas)
  • Assuntos: FISIOLOGIA
  • É parte de: Abstracts Campinas, SP
  • Notas Locais: Disponível em CD-ROM
  • Descrição: We have investigated the effects of fractions of a new type of Brazilian propolis containing phenolic acid derivatives (FRRP) on cell growth and proliferation in a human multiple myeloma cell line (RPMI 8226). The effects on cell cycle progression in the RPMI 8226 cell line were assayed with propidium iodide using DNA histograms and multiparametric flow cytometry. The inhibitory concentrations 50 (IC50) were estimated by cytostatic assay of tetrazolium (MTT) reduction. The IC50 (± S.E.M; n =3) of FRRP at 48 h exposure, was 28 ± 8 µg/ml. FRRP at a concentration of 20 µg/ml inhibited the G1/S transition of the cell cycle of the human multiple myeloma cell line, an action consistent with its cytostatic effect. Our results also revealed that mRNA expression of cyclin-dependent kinase inhibitors, p16, as assayed by RT-PCR, did not change in multiple myeloma. All procedures were performed in triplicate with appropriate controls. The chemical composition of FRRP was evaluated by High performance liquid chromatography coupled to mass detector. Our observations suggest that the FRRP from Brazilian propolis may be a powerful therapeutic drug in myeloma pathogenesis
  • Editor: Campinas
  • Data de criação/publicação: 2004
  • Formato: [s. p.].
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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