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The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line

V. M. Freitas E Oliveira; C Furuse; V. C Araújo; Ruy Gastaldoni Jaeger; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)

Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005

Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental 2005

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  • Título:
    The laminin derived peptide YIGSR modulates adhesion molecules in an adenoid cystic carcinoma cell line
  • Autor: V. M. Freitas
  • E Oliveira; C Furuse; V. C Araújo; Ruy Gastaldoni Jaeger; Reunião Anual da Federação de Sociedades de Biologia Experimental, FeSBE (20. 2005 Águas de Lindóia, SP)
  • Assuntos: HISTOLOGIA
  • É parte de: Resumos Águas de Lindóia, São Paulo: Federação de Sociedades de Biologia Experimental, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Adenoid cystic carcinoma is a frequent malignant salivary gland neoplasm with high level of recurrence and distant metastasis. We have demonstrated that laminin-1 modulates the phenotype of an adenoid cystic carcinoma (CAC2) cell line. We are currently studying the expression of a non-integrin laminin receptor, the 32/67kDa laminin-binding protein (LBP), in adenoid cystic carcinoma. Immunohistochemistry showed the presence of LBP in adenoid cystic carcinoma in vivo. Immunofluorescence of CAC2 cells also revealed this receptor. The LBP binds to a sequence of the laminin beta 1 chain, the YIGSR peptide. This peptide promotes cell attachment and migration. Thus, we decided to study the role played by the peptide YIGSR and its ligand, the LBP receptor, in CAC2 cells. These cells were grown inside either laminin-1 (controls) or laminin-1 enriched with YIGSR (treated). Light microscopy showed that laminin-1 induced CAC2 cells to create solid formations of cells closely packed. On the other hand, CAC2 cells grown in contact with YIGSR showed a completely different pattern. Cells were non-cohesive and spindle shaped. To address whether the effect of YIGSR was dependent on LBP, CAC2 cells were pretreated with antibody against this receptor. Blockage of LBP receptor inhibited YIGSR effect on CAC2 morphology. We then decided to study whether YIGSR would inhibit the presence of adhesion molecules in CAC2 cells. We prepared cytoskeletal fractions of CAC2 cells grown either
    inside laminin-1 or laminin-1 enriched with YIGSR. Western blot of these fractions demonstrated that YIGSR decreased the amount of E-cadherin and beta-catenin in CAC2 cells, when compared to cells grown inside laminin-1. Pretreatment of CAC2 cells wit anti-LBP restored e-cadherin and beta catenin expression Conclusões: YIGSR and its ligand, 32/67kDa LBP regulates the expression of e-cadherin and beta-catenin in an adenoid cystic carcinoma cell line
  • Editor: Águas de Lindóia, São Paulo Federação de Sociedades de Biologia Experimental
  • Data de criação/publicação: 2005
  • Formato: res. 42.056.
  • Idioma: Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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