Idioma:

Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles

Špačková, Barbora ; Klein Moberg, Henrik ; Fritzsche, Joachim ; Tenghamn, Johan ; Sjösten, Gustaf ; Šípová-Jungová, Hana ; Albinsson, David ; Lubart, Quentin ; van Leeuwen, Daniel ; Westerlund, Fredrik ; Midtvedt, Daniel ; Esbjörner, Elin K ; Käll, Mikael ; Volpe, Giovanni ; Langhammer, Christoph

Nature methods, 2022-06, Vol.19 (6), p.751-758 [Periódico revisado por pares]

United States: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles
Autor: Špačková, Barbora ; Klein Moberg, Henrik ; Fritzsche, Joachim ; Tenghamn, Johan ; Sjösten, Gustaf ; Šípová-Jungová, Hana ; Albinsson, David ; Lubart, Quentin ; van Leeuwen, Daniel ; Westerlund, Fredrik ; Midtvedt, Daniel ; Esbjörner, Elin K ; Käll, Mikael ; Volpe, Giovanni ; Langhammer, Christoph
Assuntos: Biochemistry & Molecular Biology ; Biochemistry and Molecular Biology ; Biokemi och molekylärbiologi ; Biomolecules ; Cell culture ; Data interpretation ; Fluidics ; Fluorescence ; Fluorescence microscopy ; membrane ; Microscopy ; Molecular weight ; Nanofluids ; Nanoparticles ; particles ; Scattering ; tracking
É parte de: Nature methods, 2022-06, Vol.19 (6), p.751-758
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated species to bind to a surface to be visible, thereby leaving a large fraction of analytes undetected. Here, we present nanofluidic scattering microscopy (NSM), which overcomes these limitations by enabling label-free, real-time imaging of single biomolecules diffusing inside a nanofluidic channel. NSM facilitates accurate determination of molecular weight from the measured optical contrast and of the hydrodynamic radius from the measured diffusivity, from which information about the conformational state can be inferred. Furthermore, we demonstrate its applicability to the analysis of a complex biofluid, using conditioned cell culture medium containing extracellular vesicles as an example. We foresee the application of NSM to monitor conformational changes, aggregation and interactions of single biomolecules, and to analyze single-cell secretomes.
Editor: United States: Nature Publishing Group
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Label-free nanofluidic scattering microscopy of size and mass of single diffusing molecules and nanoparticles

United States: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.