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Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer

Öhlund, Daniel ; Handly-Santana, Abram ; Biffi, Giulia ; Elyada, Ela ; Almeida, Ana S ; Ponz-Sarvise, Mariano ; Corbo, Vincenzo ; Oni, Tobiloba E ; Hearn, Stephen A ; Lee, Eun Jung ; Chio, Iok In Christine ; Hwang, Chang-Il ; Tiriac, Hervé ; Baker, Lindsey A ; Engle, Dannielle D ; Feig, Christine ; Kultti, Anne ; Egeblad, Mikala ; Fearon, Douglas T ; Crawford, James M ; Clevers, Hans ; Park, Youngkyu ; Tuveson, David A

The Journal of experimental medicine, 2017-03, Vol.214 (3), p.579-596 [Periódico revisado por pares]

United States: Rockefeller University Press

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Título:
Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer
Autor: Öhlund, Daniel ; Handly-Santana, Abram ; Biffi, Giulia ; Elyada, Ela ; Almeida, Ana S ; Ponz-Sarvise, Mariano ; Corbo, Vincenzo ; Oni, Tobiloba E ; Hearn, Stephen A ; Lee, Eun Jung ; Chio, Iok In Christine ; Hwang, Chang-Il ; Tiriac, Hervé ; Baker, Lindsey A ; Engle, Dannielle D ; Feig, Christine ; Kultti, Anne ; Egeblad, Mikala ; Fearon, Douglas T ; Crawford, James M ; Clevers, Hans ; Park, Youngkyu ; Tuveson, David A
Assuntos: Actin ; Actins - analysis ; Adenocarcinoma ; Animals ; Biology ; Cancer ; Carcinoma, Pancreatic Ductal - immunology ; Carcinoma, Pancreatic Ductal - metabolism ; Carcinoma, Pancreatic Ductal - pathology ; Cell culture ; Cells, Cultured ; Crosstalk ; Cytokines - biosynthesis ; Etiology ; Fibroblasts ; Fibroblasts - physiology ; Heterogeneity ; Humans ; Inflammation ; Interleukin 6 ; Mice ; Mice, Inbred C57BL ; Muscles ; Myofibroblasts - physiology ; Organoids ; Pancreatic cancer ; Pancreatic Neoplasms - immunology ; Pancreatic Neoplasms - metabolism ; Pancreatic Neoplasms - pathology ; Populations ; Rodents ; Smooth muscle ; STAT3 Transcription Factor - metabolism ; Stellate cells ; Stromal cells
É parte de: The Journal of experimental medicine, 2017-03, Vol.214 (3), p.579-596
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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D. Öhlund, A. Handly-Santana, G. Biffi, and E. Elyada contributed equally to this paper.
Descrição: Pancreatic stellate cells (PSCs) differentiate into cancer-associated fibroblasts (CAFs) that produce desmoplastic stroma, thereby modulating disease progression and therapeutic response in pancreatic ductal adenocarcinoma (PDA). However, it is unknown whether CAFs uniformly carry out these tasks or if subtypes of CAFs with distinct phenotypes in PDA exist. We identified a CAF subpopulation with elevated expression of α-smooth muscle actin (αSMA) located immediately adjacent to neoplastic cells in mouse and human PDA tissue. We recapitulated this finding in co-cultures of murine PSCs and PDA organoids, and demonstrated that organoid-activated CAFs produced desmoplastic stroma. The co-cultures showed cooperative interactions and revealed another distinct subpopulation of CAFs, located more distantly from neoplastic cells, which lacked elevated αSMA expression and instead secreted IL6 and additional inflammatory mediators. These findings were corroborated in mouse and human PDA tissue, providing direct evidence for CAF heterogeneity in PDA tumor biology with implications for disease etiology and therapeutic development.
Editor: United States: Rockefeller University Press
Idioma: Inglês

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Distinct populations of inflammatory fibroblasts and myofibroblasts in pancreatic cancer

United States: Rockefeller University Press

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