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Metabolomics analysis and metabolite‐agronomic trait associations using kernels of wheat (Triticum aestivum) recombinant inbred lines

Shi, Taotao ; Zhu, Anting ; Jia, Jingqi ; Hu, Xin ; Chen, Jie ; Liu, Wei ; Ren, Xifeng ; Sun, Dongfa ; Fernie, Alisdair R. ; Cui, Fa ; Chen, Wei

The Plant journal : for cell and molecular biology, 2020-07, Vol.103 (1), p.279-292 [Periódico revisado por pares]

England: Blackwell Publishing Ltd

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Título:
Metabolomics analysis and metabolite‐agronomic trait associations using kernels of wheat (Triticum aestivum) recombinant inbred lines
Autor: Shi, Taotao ; Zhu, Anting ; Jia, Jingqi ; Hu, Xin ; Chen, Jie ; Liu, Wei ; Ren, Xifeng ; Sun, Dongfa ; Fernie, Alisdair R. ; Cui, Fa ; Chen, Wei
Assuntos: agronomic trait ; Agronomy ; Association analysis ; Correlation analysis ; Edible Grain - metabolism ; Flavonoids ; Gene mapping ; Genes, Plant - genetics ; Genetic analysis ; Genetic Association Studies ; Genetic relationship ; Genomes ; Inbreeding ; Kernels ; Linkage analysis ; Localization ; Loci ; mature seed ; metabolic prediction ; metabolic quantitative trait loci ; Metabolism ; Metabolites ; Metabolomics ; Methylation ; Original ; Plant breeding ; Plant Breeding - methods ; Polymorphism, Single Nucleotide - genetics ; Power plants ; Quantitative trait loci ; Quantitative Trait Loci - genetics ; Quantitative Trait, Heritable ; Triticum - genetics ; Triticum - metabolism ; Triticum aestivum L ; Wheat
É parte de: The Plant journal : for cell and molecular biology, 2020-07, Vol.103 (1), p.279-292
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These authors contributed equally to this work.
Descrição: Summary Plants produce numerous metabolites that are important for their development and growth. However, the genetic architecture of the wheat metabolome has not been well studied. Here, utilizing a high‐density genetic map, we conducted a comprehensive metabolome study via widely targeted LC‐MS/MS to analyze the wheat kernel metabolism. We further combined agronomic traits and dissected the genetic relationship between metabolites and agronomic traits. In total, 1260 metabolic features were detected. Using linkage analysis, 1005 metabolic quantitative trait loci (mQTLs) were found distributed unevenly across the genome. Twenty‐four candidate genes were found to modulate the levels of different metabolites, of which two were functionally annotated by in vitro analysis to be involved in the synthesis and modification of flavonoids. Combining the correlation analysis of metabolite‐agronomic traits with the co‐localization of methylation quantitative trait locus (mQTL) and phenotypic QTL (pQTL), genetic relationships between the metabolites and agronomic traits were uncovered. For example, a candidate was identified using correlation and co‐localization analysis that may manage auxin accumulation, thereby affecting number of grains per spike (NGPS). Furthermore, metabolomics data were used to predict the performance of wheat agronomic traits, with metabolites being found that provide strong predictive power for NGPS and plant height. This study used metabolomics and association analysis to better understand the genetic basis of the wheat metabolism which will ultimately assist in wheat breeding. Significance Statement This work performed a comprehensive metabolome analysis of kernels from wheat RILs, which, in conjunction with wheat genome data, provided a valuable resource for mQTL analysis, identification of unknown enzymes, and metabolite‐agronomic trait correlations.
Editor: England: Blackwell Publishing Ltd
Idioma: Inglês

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Metabolomics analysis and metabolite‐agronomic trait associations using kernels of wheat (Triticum aestivum) recombinant inbred lines

Shi, Taotao ; Zhu, Anting ; Jia, Jingqi ; Hu, Xin ; Chen, Jie ; Liu, Wei ; Ren, Xifeng ; Sun, Dongfa ; Fernie, Alisdair R. ; Cui, Fa ; Chen, Wei

England: Blackwell Publishing Ltd

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