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Single-molecule nanopore enzymology

Willems, Kherim ; Van Meervelt, Veerle ; Wloka, Carsten ; Maglia, Giovanni

Philosophical transactions of the Royal Society of London. Series B. Biological sciences, 2017-08, Vol.372 (1726), p.20160230-20160230 [Periódico revisado por pares]

England: The Royal Society

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Título:
Single-molecule nanopore enzymology
Autor: Willems, Kherim ; Van Meervelt, Veerle ; Wloka, Carsten ; Maglia, Giovanni
Assuntos: Biological membranes ; Biosensing Techniques ; Chemical reactions ; Deoxyribonucleic acid ; DNA ; Enzymes - chemistry ; Enzymology ; Membrane proteins ; Membranes ; Membranes, Artificial ; Nanopore Enzymology ; Nanopores ; Nanotechnology - instrumentation ; Nucleotide sequence ; Porosity ; Protein Trapping ; Proteins ; Proteins - chemistry ; Review ; Sequence Analysis, DNA ; Single Molecule Imaging ; Single-Molecule
É parte de: Philosophical transactions of the Royal Society of London. Series B. Biological sciences, 2017-08, Vol.372 (1726), p.20160230-20160230
Notas: Discussion meeting issue ‘Membrane pores: from structure and assembly, to medicine and technology’ organized and edited by Robert Gilbert, Hagan Bayley and Gregor Anderluh
These authors contributed equally to this study.
One contribution of 17 to a discussion meeting issue ‘Membrane pores: from structure and assembly, to medicine and technology’.
Descrição: Biological nanopores are a class of membrane proteins that open nanoscale water conduits in biological membranes. When they are reconstituted in artificial membranes and a bias voltage is applied across the membrane, the ionic current passing through individual nanopores can be used to monitor chemical reactions, to recognize individual molecules and, of most interest, to sequence DNA. In addition, a more recent nanopore application is the analysis of single proteins and enzymes. Monitoring enzymatic reactions with nanopores, i.e. nanopore enzymology, has the unique advantage that it allows long-timescale observations of native proteins at the single-molecule level. Here, we describe the approaches and challenges in nanopore enzymology. This article is part of the themed issue ‘Membrane pores: from structure and assembly, to medicine and technology’.
Editor: England: The Royal Society
Idioma: Inglês

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Single-molecule nanopore enzymology

Willems, Kherim ; Van Meervelt, Veerle ; Wloka, Carsten ; Maglia, Giovanni

England: The Royal Society

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