Idioma:

Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer

Carvalho, S ; Catarino, T A ; Dias, A M ; Kato, M ; Almeida, A ; Hessling, B ; Figueiredo, J ; Gärtner, F ; Sanches, J M ; Ruppert, T ; Miyoshi, E ; Pierce, M ; Carneiro, F ; Kolarich, D ; Seruca, R ; Yamaguchi, Y ; Taniguchi, N ; Reis, C A ; Pinho, S S

Oncogene, 2016-03, Vol.35 (13), p.1619-1631 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer
Autor: Carvalho, S ; Catarino, T A ; Dias, A M ; Kato, M ; Almeida, A ; Hessling, B ; Figueiredo, J ; Gärtner, F ; Sanches, J M ; Ruppert, T ; Miyoshi, E ; Pierce, M ; Carneiro, F ; Kolarich, D ; Seruca, R ; Yamaguchi, Y ; Taniguchi, N ; Reis, C A ; Pinho, S S
Assuntos: Adherens junctions ; Amino Acid Sequence ; Animal models ; Animals ; Asparagine ; Asparagine - genetics ; Cadherins ; Cadherins - chemistry ; Cadherins - genetics ; Cadherins - metabolism ; Cadherins - physiology ; Carcinogenesis ; Carcinoma ; Catalytic Domain - genetics ; Cell adhesion ; Cell adhesion & migration ; Cell aggregation ; Cell Line, Tumor ; Development and progression ; Dogs ; E-cadherin ; Gastric cancer ; Gastric mucosa ; Gastric Mucosa - metabolism ; Gastric Mucosa - pathology ; Gene expression ; Genetic aspects ; Glycosylation ; Health aspects ; HT29 Cells ; Humans ; Localization ; Madin Darby Canine Kidney Cells ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; N-Acetylglucosaminyltransferase V ; N-Acetylglucosaminyltransferases - antagonists & inhibitors ; N-Acetylglucosaminyltransferases - genetics ; N-Acetylglucosaminyltransferases - metabolism ; N-Acetylglucosinyldiphosphodolichol N-acetylglucosaminyltransferase ; N-glycans ; Polysaccharides ; Properties ; Protein expression ; Sequence Homology, Amino Acid ; Stomach cancer ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Transgenic mice ; Tumor suppression ; Tumorigenesis ; Tumors
É parte de: Oncogene, 2016-03, Vol.35 (13), p.1619-1631
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: E-cadherin is a central molecule in the process of gastric carcinogenesis and its posttranslational modifications by N-glycosylation have been described to induce a deleterious effect on cell adhesion associated with tumor cell invasion. However, the role that site-specific glycosylation of E-cadherin has in its defective function in gastric cancer cells needs to be determined. Using transgenic mice models and human clinical samples, we demonstrated that N-acetylglucosaminyltransferase V (GnT-V)-mediated glycosylation causes an abnormal pattern of E-cadherin expression in the gastric mucosa. In vitro models further indicated that, among the four potential N-glycosylation sites of E-cadherin, Asn-554 is the key site that is selectively modified with β1,6 GlcNAc-branched N-glycans catalyzed by GnT-V. This aberrant glycan modification on this specific asparagine site of E-cadherin was demonstrated to affect its critical functions in gastric cancer cells by affecting E-cadherin cellular localization, cis-dimer formation, molecular assembly and stability of the adherens junctions and cell-cell aggregation, which was further observed in human gastric carcinomas. Interestingly, manipulating this site-specific glycosylation, by preventing Asn-554 from receiving the deleterious branched structures, either by a mutation or by silencing GnT-V, resulted in a protective effect on E-cadherin, precluding its functional dysregulation and contributing to tumor suppression.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Preventing E-cadherin aberrant N-glycosylation at Asn-554 improves its critical function in gastric cancer

Carvalho, S ; Catarino, T A ; Dias, A M ; Kato, M ; Almeida, A ; Hessling, B ; Figueiredo, J ; Gärtner, F ; Sanches, J M ; Ruppert, T ; Miyoshi, E ; Pierce, M ; Carneiro, F ; Kolarich, D ; Seruca, R ; Yamaguchi, Y ; Taniguchi, N ; Reis, C A ; Pinho, S S

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.