Idioma:

Vaspin Is an Adipokine Ameliorating ER Stress in Obesity as a Ligand for Cell-Surface GRP78/MTJ-1 Complex

NAKATSUKA, Atsuko ; WADA, Jun ; HIDA, Kazuyuki ; EGUCHI, Jun ; HORIGUCHI, Chikage Sato ; OGAWA, Daisuke ; MATSUKI, Yasushi ; HIRAMATSU, Ryuji ; YAGITA, Hideo ; KAKUTA, Shigeru ; IWAKURA, Yoichiro ; MAKINO, Hirofumi ; ISEDA, Izumi ; TESHIGAWARA, Sanae ; HIGASHIO, Kanji ; MURAKAMI, Kazutoshi ; KANZAKI, Motoko ; INOUE, Kentaro ; TERAMI, Takahiro ; KATAYAMA, Akihiro

Diabetes (New York, N.Y.), 2012-11, Vol.61 (11), p.2823-2832 [Periódico revisado por pares]

Alexandria, VA: American Diabetes Association

Texto completo disponível

Citações Citado por

Enviar para

Título:
Vaspin Is an Adipokine Ameliorating ER Stress in Obesity as a Ligand for Cell-Surface GRP78/MTJ-1 Complex
Autor: NAKATSUKA, Atsuko ; WADA, Jun ; HIDA, Kazuyuki ; EGUCHI, Jun ; HORIGUCHI, Chikage Sato ; OGAWA, Daisuke ; MATSUKI, Yasushi ; HIRAMATSU, Ryuji ; YAGITA, Hideo ; KAKUTA, Shigeru ; IWAKURA, Yoichiro ; MAKINO, Hirofumi ; ISEDA, Izumi ; TESHIGAWARA, Sanae ; HIGASHIO, Kanji ; MURAKAMI, Kazutoshi ; KANZAKI, Motoko ; INOUE, Kentaro ; TERAMI, Takahiro ; KATAYAMA, Akihiro
Assuntos: Adipokines - chemistry ; Adipokines - genetics ; Adipokines - isolation & purification ; Adipokines - metabolism ; Analysis ; Animals ; Biological and medical sciences ; Cell Line, Tumor ; Cell Membrane - metabolism ; Diabetes. Impaired glucose tolerance ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Endoplasmic reticulum ; Endoplasmic Reticulum Stress ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Heat-Shock Proteins - antagonists & inhibitors ; Heat-Shock Proteins - genetics ; Heat-Shock Proteins - metabolism ; Hepatocytes - metabolism ; Hepatocytes - pathology ; HSP40 Heat-Shock Proteins - antagonists & inhibitors ; HSP40 Heat-Shock Proteins - genetics ; HSP40 Heat-Shock Proteins - metabolism ; Humans ; Intra-Abdominal Fat - metabolism ; Ligands ; Male ; Medical sciences ; Metabolic diseases ; Mice ; Mice, Knockout ; Mice, Transgenic ; Neoplasm Proteins - antagonists & inhibitors ; Neoplasm Proteins - genetics ; Neoplasm Proteins - metabolism ; Obesity ; Obesity - metabolism ; Obesity - pathology ; Obesity Studies ; Physiological aspects ; Protease inhibitors ; Protein Transport ; Rats ; Recombinant Proteins - antagonists & inhibitors ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; Serpins - chemistry ; Serpins - genetics ; Serpins - isolation & purification ; Serpins - metabolism ; Signal Transduction ; Up-Regulation
É parte de: Diabetes (New York, N.Y.), 2012-11, Vol.61 (11), p.2823-2832
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: It is unknown whether adipokines derived from adipose tissues modulate endoplasmic reticulum (ER) stress induced in obesity. Here, we show that visceral adipose tissue-derived serine protease inhibitor (vaspin) binds to cell-surface 78-kDa glucose-regulated protein (GRP78), which is recruited from ER to plasma membrane under ER stress. Vaspin transgenic mice were protected from diet-induced obesity, glucose intolerance, and hepatic steatosis, while vaspin-deficient mice developed glucose intolerance associated with upregulation of ER stress markers. With tandem affinity tag purification using HepG2 cells, we identified GRP78 as an interacting molecule. The complex formation of vaspin, GRP78, and murine tumor cell DnaJ-like protein 1 (MTJ-1) (DnaJ homolog, subfamily C, member 1) on plasma membrane was confirmed by cell-surface labeling with biotin and immunoprecipitation in liver tissues and H-4-II-E-C3 cells. The addition of recombinant human vaspin in the cultured H-4-II-E-C3 cells also increased the phosphorylation of Akt and AMP-activated protein kinase (AMPK) in a dose-dependent manner, and anti-GRP78 antibodies completely abrogated the vaspin-induced upregulation of pAkt and pAMPK. Vaspin is a novel ligand for cell-surface GRP78/MTJ-1 complex, and its subsequent signals exert beneficial effects on ER stress-induced metabolic dysfunctions.
Editor: Alexandria, VA: American Diabetes Association
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Vaspin Is an Adipokine Ameliorating ER Stress in Obesity as a Ligand for Cell-Surface GRP78/MTJ-1 Complex

Alexandria, VA: American Diabetes Association

Buscando em bases de dados remotas. Favor aguardar.