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MSCs alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of macrophages in mouse lung organoid–macrophage model

Zhu, Jiaqi ; Zhou, Jiahang ; Feng, Bing ; Pan, Qiaoling ; Yang, Jinfeng ; Lang, Guanjing ; Shang, Dandan ; Zhou, Jianya ; Li, Lanjuan ; Yu, Jiong ; Cao, Hongcui

Cellular and molecular life sciences : CMLS, 2024-12, Vol.81 (1), p.124-124 [Periódico revisado por pares]

Cham: Springer International Publishing

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Título:
MSCs alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of macrophages in mouse lung organoid–macrophage model
Autor: Zhu, Jiaqi ; Zhou, Jiahang ; Feng, Bing ; Pan, Qiaoling ; Yang, Jinfeng ; Lang, Guanjing ; Shang, Dandan ; Zhou, Jianya ; Li, Lanjuan ; Yu, Jiong ; Cao, Hongcui
Assuntos: Acute Lung Injury - chemically induced ; Acute Lung Injury - therapy ; Animals ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Bronchus ; Cell activation ; Cell Biology ; Cell culture ; Chemotaxis ; Clinical trials ; Cytokines ; Disease Models, Animal ; Edema ; External stimuli ; Gene expression ; Gene sequencing ; Health services ; Hospitals ; Immune system ; Immunomodulation ; Infectious diseases ; Inflammation ; Inflammation - metabolism ; Inflammation - therapy ; Inflammatory diseases ; Injury analysis ; Injury prevention ; Laboratories ; Lavage ; Life Sciences ; Lipopolysaccharides ; Lipopolysaccharides - pharmacology ; Lung - metabolism ; Lungs ; Macrophages ; Macrophages - metabolism ; Macrophages, Alveolar - metabolism ; Medical diagnosis ; Medicine ; Mesenchymal Stem Cells ; Mice ; Organoids ; Organoids - metabolism ; Original ; Original Article ; Penicillin ; Phenotypes ; Pneumonia ; Spreading ; Stem cells ; Transcriptomes
É parte de: Cellular and molecular life sciences : CMLS, 2024-12, Vol.81 (1), p.124-124
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Acute lung injury (ALI) is an inflammatory disease associated with alveolar injury, subsequent macrophage activation, inflammatory cell infiltration, and cytokine production. Mesenchymal stem cells (MSCs) are beneficial for application in the treatment of inflammatory diseases due to their immunomodulatory effects. However, the mechanisms of regulatory effects by MSCs on macrophages in ALI need more in-depth study. Lung tissues were collected from mice for mouse lung organoid construction. Alveolar macrophages (AMs) derived from bronchoalveolar lavage and interstitial macrophages (IMs) derived from lung tissue were co-cultured, with novel matrigel-spreading lung organoids to construct an in vitro model of lung organoids–immune cells. Mouse compact bone-derived MSCs were co-cultured with organoids–macrophages to confirm their therapeutic effect on acute lung injury. Changes in transcriptome expression profile were analyzed by RNA sequencing. Well-established lung organoids expressed various lung cell type-specific markers. Lung organoids grown on spreading matrigel had the property of functional cells growing outside the lumen. Lipopolysaccharide (LPS)-induced injury promoted macrophage chemotaxis toward lung organoids and enhanced the expression of inflammation-associated genes in inflammation-injured lung organoids–macrophages compared with controls. Treatment with MSCs inhibited the injury progress and reduced the levels of inflammatory components. Furthermore, through the nuclear factor-κB pathway, MSC treatment inhibited inflammatory and phenotypic transformation of AMs and modulated the antigen-presenting function of IMs, thereby affecting the inflammatory phenotype of lung organoids. Lung organoids grown by spreading matrigel facilitate the reception of external stimuli and the construction of in vitro models containing immune cells, which is a potential novel model for disease research. MSCs exert protective effects against lung injury by regulating different functions of AMs and IMs in the lung, indicating a potential mechanism for therapeutic intervention.
Editor: Cham: Springer International Publishing
Idioma: Inglês

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MSCs alleviate LPS-induced acute lung injury by inhibiting the proinflammatory function of macrophages in mouse lung organoid–macrophage model

Zhu, Jiaqi ; Zhou, Jiahang ; Feng, Bing ; Pan, Qiaoling ; Yang, Jinfeng ; Lang, Guanjing ; Shang, Dandan ; Zhou, Jianya ; Li, Lanjuan ; Yu, Jiong ; Cao, Hongcui

Cham: Springer International Publishing

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