Idioma:

Mutagenesis; Research on mutagenesis discussed by scientists at University of Cincinnati, College of Medicine

Life Science Weekly, 2010, p.3522

Texto completo disponível

Citações Citado por

Título:
Mutagenesis; Research on mutagenesis discussed by scientists at University of Cincinnati, College of Medicine
É parte de: Life Science Weekly, 2010, p.3522
Descrição: Understanding molecular mechanisms regulating mating in this organism may allow us to reverse or prevent loss of mating in H. capsulatum strains, introducing a variety of classical genetics techniques to the field. We generated a strain, UC1, by insertional mutagenesis of the laboratory strain G217B, and found that UC1 acquired the ability to form mating structures called cleistothecia. The aim of this study was to determine the mechanism by which UC1 gained the ability to form cleistothecia. We also present initial studies demonstrating that UC1 can be used as a tool to determine molecular correlates of mating in H. capsulatum. The strain UC1 was found to have increased RNA levels of the mating locus transcription factor (MAT1-1-1), and the putative alpha pheromone (PPG1) compared to G217B. Agrobacterium-mediated transformation and integration of T-DNA from the vector pCB301-GFP-HYG were found to be partially responsible for the increased RNA levels of these genes; however, the site of integration appeared to play the largest role in the strain's ability to form cleistothecia.
Editor: Atlanta: NewsRx
Idioma: Inglês

Voltar para lista de resultados

Buscando em bases de dados remotas. Favor aguardar.