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Dissociation by cytochalasin B of movement, DNA synthesis and transport in 3T3 cells

Brownstein, Barbara L. ; Rozengurt, Enrique ; de Asua, Luis Jimenez ; Stoker, Michael

Journal of cellular physiology, 1975-06, Vol.85 (3), p.579-585 [Periódico revisado por pares]

Hoboken: Wiley Subscription Services, Inc., A Wiley Company

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  • Título:
    Dissociation by cytochalasin B of movement, DNA synthesis and transport in 3T3 cells
  • Autor: Brownstein, Barbara L. ; Rozengurt, Enrique ; de Asua, Luis Jimenez ; Stoker, Michael
  • Assuntos: Animals ; Biological Transport - drug effects ; Blood ; Cell Line ; Cell Movement - drug effects ; Cells, Cultured ; Cytochalasin B - pharmacology ; Dimethyl Sulfoxide - pharmacology ; DNA - biosynthesis ; Glucose - metabolism ; Mice ; Mice, Inbred BALB C ; Phosphates - metabolism ; Thymidine - metabolism ; Uridine - metabolism
  • É parte de: Journal of cellular physiology, 1975-06, Vol.85 (3), p.579-585
  • Notas: ArticleID:JCP1040850309
    istex:EB27993C9E7644F4528FD7143DC01AAD4F457A2C
    ark:/67375/WNG-0FLZ71GH-K
    Department of Biology, Temple University, Philadelphia, Pa. 19122
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Cytochalasin B was used as a tool to study the inter‐relationships between cell movement, the reinitiated DNA synthesis and the enhanced transport of specific small molecules stimulated by serum in quiescent 3T3 cells. Cytochalasin at concentrations of less than 1 μg/ml inhibits serum‐stimulated movement within the monolayer and migration into a wound. Even at ten times this concentration there is little effect on the increase in DNA in the culture, indicating that movement away from neighboring cells is not required for the initiation of DNA synthesis. While DNA synthesis is not inhibited by concentrations of cytochalasin up to 10 μg/ml, the increased thymidine transport which is associated with the onset of the S phase of the cell cycle is inhibited and DNA synthesis cannot be measured by the labelling of nuclei with radioactive thymidine. Cytochalasin has a differential effect on the early transport changes produced by serum addition. Glucose transport is inhibited by low concentrations of the drug (< 1 μg/ml) while the enhanced uptake of phosphate and uridine is unaffected by a 10‐fold increase in concentration. Although the doses of cytochalasin required for 50% inhibition of hexose uptake and of cell movement are the same, no causal relationship between sugar transport and locomotion can be demonstrated. Cytochalasin affects membrane functions in at least two different ways. The drug inhibits the uptake of glucose directly but affects only the S‐phase associated increase in thymidine transport.
  • Editor: Hoboken: Wiley Subscription Services, Inc., A Wiley Company
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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