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Induction of β-casein mRNA accumulation by the putative prolactin second messenger added to the culture medium of cultured mammary epithelial cells

Servely, J.L. ; Teyssot, B. ; Houdebine, L.M. ; Delouis, C. ; Djiane, J. ; Kelly, P.A.

FEBS letters, 1982-11, Vol.148 (2), p.242-246 [Periódico revisado por pares]

England: Elsevier B.V

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Título:
Induction of β-casein mRNA accumulation by the putative prolactin second messenger added to the culture medium of cultured mammary epithelial cells
Autor: Servely, J.L. ; Teyssot, B. ; Houdebine, L.M. ; Delouis, C. ; Djiane, J. ; Kelly, P.A.
Assuntos: Animals ; Caseins - genetics ; cDNA probe ; Cells, Cultured ; Chorionic Gonadotropin - pharmacology ; Colchicine - pharmacology ; Epithelium - metabolism ; Female ; Follicle Stimulating Hormone - pharmacology ; Mammary cell culture ; Mammary Glands, Animal - drug effects ; Mammary Glands, Animal - metabolism ; Microsome ; Microsomes - metabolism ; Pregnancy ; Prolactin ; Prolactin - pharmacology ; Pseudopregnancy - metabolism ; Rabbits ; RNA, Messenger - genetics ; Second messenger ; Transcription, Genetic - drug effects ; β-Casein mRNA
É parte de: FEBS letters, 1982-11, Vol.148 (2), p.242-246
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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Descrição: It has been observed that the incubation of mammary membranes with prolactin provokes the release of a factor which is a potent stimulator of beta -casein gene transcription when added to isolated nuclei. This factor is specifically generated by lactogenic hormones and it stimulates at least one of the prolactin-sensitive genes. The active fraction liberated from mammary membranes by prolactin has a small M sub(r)-value. Here, the supernatant from membranes which contain the putative prolactin second messenger were added to the culture medium of isolated mammary epithelial cells, in an attempt to provoke the accumulation of casein rRNA and mimic prolactin action and in order to overcome possible problems encountered with the use of isolated nuclei.
Editor: England: Elsevier B.V
Idioma: Inglês

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Induction of β-casein mRNA accumulation by the putative prolactin second messenger added to the culture medium of cultured mammary epithelial cells

Servely, J.L. ; Teyssot, B. ; Houdebine, L.M. ; Delouis, C. ; Djiane, J. ; Kelly, P.A.

England: Elsevier B.V

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