Idioma:

Chemical ischemia' in cultured retina cells: the role of excitatory amino acid receptors and of energy levels on cell death

Ferreira, Ildete L ; Duarte, Carlos B ; Carvalho, Arsélio P

Brain research, 1997-09, Vol.768 (1), p.157-166 [Periódico revisado por pares]

Netherlands: Elsevier B.V

Texto completo disponível

Citações Citado por

Enviar para

Título:
Chemical ischemia' in cultured retina cells: the role of excitatory amino acid receptors and of energy levels on cell death
Autor: Ferreira, Ildete L ; Duarte, Carlos B ; Carvalho, Arsélio P
Assuntos: Adenine Nucleotides - metabolism ; Amino acid release ; Amino Acids - metabolism ; Animals ; Cell Death - drug effects ; Cells, Cultured ; Chick Embryo ; Energy level ; Energy Metabolism - drug effects ; Excitatory Amino Acid Antagonists - pharmacology ; gamma-Aminobutyric Acid - metabolism ; Glutamate receptor ; Ischemia ; Ischemia - chemically induced ; Membrane Potentials - drug effects ; Potassium Channels - drug effects ; Receptors, Glutamate - physiology ; Retina cell damage ; Retinal Vessels - drug effects ; Retinal Vessels - metabolism
É parte de: Brain research, 1997-09, Vol.768 (1), p.157-166
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: In this study, we determined whether the retina cell death observed in response to an ischemic-like insult is related to an overactivation of the ionotropic glutamate receptors and/or to a collapse of the energy levels. Cultured chick retina cells were submitted to `chemical ischemia' by metabolic inhibition with sodium cyanide and iodoacetic acid, which block oxidative phosphorylation and glycolysis, respectively. The assessment of neuronal injury was made spectrophotometrically by quantification of cellularly reduced MTT, which gives information about mitochondrial function, or by staining with fluorescein diacetate (FDA), which correlates with changes in the plasma membrane permeability. `Chemical ischemia' induced both an acute and a delayed time-dependent degeneration of chick retina cells. We observed that 2 min after the ischemic insult, the levels of ATP were reduced to a minimum. On the other hand, the metabolic inhibition induced the release of aspartate, glutamate and γ-aminobutyric acid, and the activation of AMPA/kainate receptors during the period of metabolic arrest was partially responsible for the loss of mitochondrial function. However, the NMDA and non-NMDA receptor antagonists (MK-801 and CNQX) did not prevent the plasma membrane damage caused by sodium cyanide and iodoacetic acid. The results show that the collapse of the energy levels, rather than the increase in excitatory amino acids, appears to underlie the observed cell injury, suggesting an important relationship between ischemia-induced depletion of high-energy metabolites and retina cell degeneration.
Editor: Netherlands: Elsevier B.V
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Chemical ischemia' in cultured retina cells: the role of excitatory amino acid receptors and of energy levels on cell death

Ferreira, Ildete L ; Duarte, Carlos B ; Carvalho, Arsélio P

Netherlands: Elsevier B.V

Buscando em bases de dados remotas. Favor aguardar.