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Shear Stress-induced [Ca2+]i Transients and Oscillations in Mouse Fibroblasts Are Mediated by Endogenously Released ATP (∗)

Grierson, Jeremy P. ; Meldolesi, Jacopo

The Journal of biological chemistry, 1995-03, Vol.270 (9), p.4451-4456 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Shear Stress-induced [Ca2+]i Transients and Oscillations in Mouse Fibroblasts Are Mediated by Endogenously Released ATP (∗)
  • Autor: Grierson, Jeremy P. ; Meldolesi, Jacopo
  • Assuntos: Adenosine Triphosphate - physiology ; Animals ; Apyrase - pharmacology ; Biomechanical Phenomena ; Calcium - metabolism ; Estrenes - pharmacology ; Homeostasis ; Hydrolysis ; Inositol 1,4,5-Trisphosphate - metabolism ; L Cells ; Mice ; Pyrrolidinones - pharmacology ; Sodium Chloride - pharmacology
  • É parte de: The Journal of biological chemistry, 1995-03, Vol.270 (9), p.4451-4456
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: The effects of ATP, U-73122, apyrase, and saline shear stress on [Ca2+]i homeostasis were studied in fura-2 loaded, mouse fibroblast cells (L929), both in suspension and plated on glass. Release of internal Ca2+ was induced by ATP, via a receptor identified pharmacologically as a P2U type. In single cells, low concentrations of ATP evoked [Ca2+]i oscillations. These events were blocked by the putative phospholipase C inhibitor, U-73122 (but not by the inactive analog U-73343) and by the ATP/ADPase, apyrase. In addition, both these agents reduced the [Ca2+]i of unstimulated cells, especially after stirring, and blocked spontaneously occurring [Ca2+]i oscillations, which suggested an already activated state of the ATP receptor, independent from exogenous stimulations. Moreover, it was found that stirring of the cells was correlated with a steady accumulation of inositol phosphates, also blockable by apyrase, and that [Ca2+]i mobilization could be induced by puffs of saline in single cells. The transition to a Ca2+-free environment also provoked [Ca2+]i oscillations, most likely via the increase in ATP4− concentration. This evidence suggests that endogenous ATP is released from L fibroblasts in response to fluid shear stress, and this results in an autocrine, tonic up-regulation of the phosphoinositide signaling system and an ensuing alteration in Ca2+ homeostasis. Up until now, such a response to shear stress was believed to be unique to endothelial cells.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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