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Expression of exo-polygalacturonases in Botrytis cinerea

Rha, E ; Park, H.J ; Kim, M.O ; Chung, Y.R ; Lee, C.W ; Kim, J.W

FEMS microbiology letters, 2001-07, Vol.201 (1), p.105-109 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Expression of exo-polygalacturonases in Botrytis cinerea
Autor: Rha, E ; Park, H.J ; Kim, M.O ; Chung, Y.R ; Lee, C.W ; Kim, J.W
Assuntos: Acids ; Antibodies ; Bacteriology ; Biological and medical sciences ; Botrytis - enzymology ; Botrytis - growth & development ; Botrytis cinerea ; carbon ; Carbon sources ; Cell culture ; Cell walls ; Citrus ; cucumbers ; Cucumis sativus - microbiology ; Culture Media ; Enzyme Induction ; Exo‐polygalacturonase ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Fungi ; galacturonic acid ; Genetics ; Glycoside Hydrolases - analysis ; Glycoside Hydrolases - biosynthesis ; Glycoside Hydrolases - immunology ; Glycoside Hydrolases - isolation & purification ; gray mold ; Grey mold ; Hexuronic Acids - pharmacology ; Host plants ; Immune Sera ; Immunoblotting ; Immunoenzyme Techniques ; immunohistochemistry ; Inoculation ; Leaves ; Maceration ; Microbiology ; molecular weight ; Pathogenesis ; Pectin ; pectins ; Pectins - pharmacology ; Phytopathology. Animal pests. Plant and forest protection ; Plant Leaves - microbiology ; Plant tissues ; polyclonal antibodies ; Polygalacturonase ; Polygalacturonic acid ; rabbits ; Spores ; Systematics. Morphology. Development cycle. Physiology
É parte de: FEMS microbiology letters, 2001-07, Vol.201 (1), p.105-109
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Descrição: The pathogenic fungus, Botrytis cinerea, causing gray mold disease in a variety of plant species, secretes at least four polygalacturonases (PGs), cell wall degrading enzymes. Among them, we prepared polyclonal antibody against purified 66-kDa exo-PG in rabbit. Immunoblot analysis revealed that the antibody recognized two exo-PGs, 66 kDa and 70 kDa in molecular mass, secreted from B. cinerea cultured in the medium containing citrus pectin as a carbon source. By immunohistochemical analysis, the expression of exo-PGs was identified in cucumber leaves inoculated with spores of B. cinerea. The exo-PGs were observed 9 h after inoculation, and the amount of exo-PGs increased with time in the leaves. The exo-PGs were induced by polygalacturonic acid as well as its monomer, galacturonic acid, in vitro. The expression of 66-kDa exo-PG (exo-PG I) increased with time of culture, while 70-kDa exo-PG (exo-PG II) was transiently expressed soon after the start of culture. Therefore, exo-PGs might play an important role in pathogenesis at an early stage of infection as well as in tissue maceration of host plant.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Expression of exo-polygalacturonases in Botrytis cinerea

Rha, E ; Park, H.J ; Kim, M.O ; Chung, Y.R ; Lee, C.W ; Kim, J.W

Oxford, UK: Blackwell Publishing Ltd

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