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Molecular characterization of caprine adeno-associated virus (AAV-Go.1) reveals striking similarity to human AAV5

Qiu, Jianming ; Cheng, Fang ; Pintel, David

Virology (New York, N.Y.), 2006-12, Vol.356 (1), p.208-216 [Periódico revisado por pares]

United States: Elsevier Inc

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Título:
Molecular characterization of caprine adeno-associated virus (AAV-Go.1) reveals striking similarity to human AAV5
Autor: Qiu, Jianming ; Cheng, Fang ; Pintel, David
Assuntos: Adeno-associated virus ; Animals ; beta-Galactosidase - genetics ; beta-Galactosidase - metabolism ; Cattle ; Cell Line ; Cercopithecus aethiops ; COS Cells ; Cricetinae ; Dependovirus - classification ; Dependovirus - genetics ; Dependovirus - pathogenicity ; Genome, Viral ; Goats - virology ; HeLa Cells ; Human adenovirus ; Humans ; Molecular Sequence Data ; Recombination, Genetic ; Sequence Analysis, DNA ; Transduction, Genetic
É parte de: Virology (New York, N.Y.), 2006-12, Vol.356 (1), p.208-216
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The complete genome of the caprine adeno-associated virus (AAV-Go.1) was sequenced, and the expression profile of AAV-Go.1 was determined following virus infection in primary lamb kidney cells. A remarkable similarity between the Rep coding region, the ITR sequence and the central intron of AAV-Go.1, and the human derived AAV5 was observed. The transcription profile of AAV-Go.1 was also quite similar to that of AAV5. AAV-Go.1 was able to efficiently infect human cell lines, following co-infection of human adenovirus, and in reciprocal experiments, AAV5 also was able to efficiently infect primary lamb cells and bovine cell lines. Recombinant AAV5 and AAV-Go.1 expressing the β-gal gene flanked by AAV5 ITRs, showed similar transduction efficiency in various human and animal cells. Studies of AAV-Go.1 may expand our understanding of the evolutionary relationship between the AAV-5-like group of AAVs, which, in addition to human derived AAV5, includes AAV viruses from bovine, caprine, and avian species.
Editor: United States: Elsevier Inc
Idioma: Inglês

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Molecular characterization of caprine adeno-associated virus (AAV-Go.1) reveals striking similarity to human AAV5

Qiu, Jianming ; Cheng, Fang ; Pintel, David

United States: Elsevier Inc

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