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Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance

Li, Bo ; Wang, Chunyan

Analyst (London), 2024-02, Vol.149 (4), p.15-154 [Periódico revisado por pares]

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Título:
Endonuclease IV and T4 ligase enhanced detection of mutations in low abundance
Autor: Li, Bo ; Wang, Chunyan
Assuntos: Deoxyribonuclease IV (Phage T4-Induced) ; DNA - analysis ; DNA - genetics ; DNA Ligases ; Ligases ; Mutation
É parte de: Analyst (London), 2024-02, Vol.149 (4), p.15-154
Notas: Electronic supplementary information (ESI) available: Materials and methods, Tables S1 and S2, and Fig. S1 and S2. See DOI
https://doi.org/10.1039/d3an02083e
ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: We propose a mutant detection approach based on endonuclease IV and DNA ligase in combination with qPCR. The enzymes functioned cooperatively to facilitate PCR for low abundance DNA detection. We demonstrate that our approach can distinguish mutations as low as 0.01%, indicating the potential application of this strategy in early cancer diagnosis. Single base mutations on a dsDNA substrate with an AP site incorporated could be easily detected through a one-pot reaction of enzymes.
Editor: England: Royal Society of Chemistry
Idioma: Inglês

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