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A new Piper nigrum cysteine proteinase inhibitor, PnCPI, with antifungal activity: molecular cloning, recombinant expression, functional analyses and molecular modeling

Lima, Aline Medeiros ; Barros, Nicolle Louise Ferreira ; Freitas, Ana Camila Oliveira ; Tavares, Liliane Souza Conceição ; Pirovani, Carlos Priminho ; Siqueira, Andrei Santos ; Gonçalves, Evonnildo Costa ; de Souza, Cláudia Regina Batista

Planta, 2020-08, Vol.252 (2), p.16-16, Article 16 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
A new Piper nigrum cysteine proteinase inhibitor, PnCPI, with antifungal activity: molecular cloning, recombinant expression, functional analyses and molecular modeling
Autor: Lima, Aline Medeiros ; Barros, Nicolle Louise Ferreira ; Freitas, Ana Camila Oliveira ; Tavares, Liliane Souza Conceição ; Pirovani, Carlos Priminho ; Siqueira, Andrei Santos ; Gonçalves, Evonnildo Costa ; de Souza, Cláudia Regina Batista
Assuntos: Agriculture ; Amino acids ; Antifungal activity ; Antifungal Agents - isolation & purification ; Antifungal Agents - pharmacology ; Biomedical and Life Sciences ; Cloning ; Cloning, Molecular ; Conserved sequence ; Cystatins ; Cystatins - pharmacology ; Cysteine ; Cysteine proteinase ; Cysteine Proteinase Inhibitors - isolation & purification ; Cysteine Proteinase Inhibitors - pharmacology ; DNA, Complementary - genetics ; E coli ; Ecology ; Forestry ; Fungicides ; Fusarium - drug effects ; Fusarium - enzymology ; Glycine ; Heat treatment ; Heat treatments ; Introns ; Life Sciences ; Modelling ; Molecular modelling ; Original Article ; Papain ; Papain - antagonists & inhibitors ; Piper nigrum ; Piper nigrum - chemistry ; Piper nigrum - genetics ; Plant Diseases - microbiology ; Plant Diseases - prevention & control ; Plant Sciences ; Proteinase ; Proteinase inhibitors ; Residues ; Root rot ; Tryptophan
É parte de: Planta, 2020-08, Vol.252 (2), p.16-16, Article 16
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SourceType-Scholarly Journals-1
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Descrição: Main conclusion A new Piper nigrum cysteine proteinase inhibitor, PnCPI, belonging to group I of phytocystatins, with inhibitory activity against papain and growth of Fusarium solani f. sp. piperis , was isolated and characterized. Previous studies (de Souza et al. 2011) have identified a partial cDNA sequence of putative cysteine proteinase inhibitor differentially expressed in roots of black pepper ( P. nigrum L.) infected by F. solani f. sp. piperis . Here, we aimed to isolate the full-length cDNA and genomic sequences of the P. nigrum cysteine proteinase inhibitor gene, named PnCPI . Sequence analyses showed that the PnCPI gene encodes a deduced protein of 108 amino acid residues with a predicted molecular mass of 12.3 kDa and isoelectric point of 6.51. Besides the LARFAV-like sequence, common to all phytocystatins, PnCPI contains three conserved motifs of the superfamily cystatin: a glycine residue at the N-terminal region, the QxVxG reactive site more centrally positioned, and one tryptophan in the C-terminal region. PnCPI, belonging to group I of phytocystatins, showed high identity with cystatins isolated from several plant species. Sequence analyses also revealed no putative signal peptide at the N-terminal of PnCPI, as well as no introns within the genomic sequence corresponding to the PnCPI coding region. Molecular modeling showed the ability of PnCPI to interact with papain, while its inhibitory activity against this protease was confirmed after heterologous expression in Escherichia coli . The effects of heat treatments on the inhibitory activity of recombinant PnCPI, rPnCPI, were evaluated. In addition, rPnCPI exhibited in vitro activity against F. solani f. sp. piperis , revealing a new cystatin with the potential antifungal application. The identification of PnCPI as a functional cystatin able to inhibit the in vitro growth of F. solani f. sp. piperis indicates other factors contributing to in vivo susceptibility of black pepper to root rot disease.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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A new Piper nigrum cysteine proteinase inhibitor, PnCPI, with antifungal activity: molecular cloning, recombinant expression, functional analyses and molecular modeling

Lima, Aline Medeiros ; Barros, Nicolle Louise Ferreira ; Freitas, Ana Camila Oliveira ; Tavares, Liliane Souza Conceição ; Pirovani, Carlos Priminho ; Siqueira, Andrei Santos ; Gonçalves, Evonnildo Costa ; de Souza, Cláudia Regina Batista

Berlin/Heidelberg: Springer Berlin Heidelberg

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