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Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases

Nemoto, Michiko ; Iwaki, Sayako ; Moriya, Hisao ; Monden, Yuki ; Tamura, Takashi ; Inagaki, Kenji ; Mayama, Shigeki ; Obuse, Kiori

Marine biotechnology (New York, N.Y.), 2020-08, Vol.22 (4), p.551-563 [Periódico revisado por pares]

New York: Springer US

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Título:
Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases
Autor: Nemoto, Michiko ; Iwaki, Sayako ; Moriya, Hisao ; Monden, Yuki ; Tamura, Takashi ; Inagaki, Kenji ; Mayama, Shigeki ; Obuse, Kiori
Assuntos: Analysis ; Bacillariophyceae ; Biomedical and Life Sciences ; Cell Wall - genetics ; Cell Wall - metabolism ; Cell walls ; Diatoms ; Diatoms - genetics ; Diatoms - metabolism ; Domains ; Endoplasmic reticulum ; Engineering ; Freshwater & Marine Ecology ; Functional materials ; Gene expression ; Genes ; Genomes ; Homology ; Identification ; Life Sciences ; Marine microorganisms ; Methyltransferase ; Microbiology ; Molecules ; Nitzschia palea ; Original Article ; Plankton ; Polyamines ; PR-SET Domains ; Protein Methyltransferases - metabolism ; Proteins ; Proteomics ; Silica ; Silicon dioxide ; Silicon Dioxide - chemistry ; Species ; Transcriptome ; Zoology
É parte de: Marine biotechnology (New York, N.Y.), 2020-08, Vol.22 (4), p.551-563
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SourceType-Scholarly Journals-1
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Descrição: Silica cell walls of diatoms have attracted attention as a source of nanostructured functional materials and have immense potential for a variety of applications. Previous studies of silica cell wall formation have identified numerous involved proteins, but most of these proteins are species-specific and are not conserved among diatoms. However, because the basic process of diatom cell wall formation is common to all diatom species, ubiquitous proteins and molecules will reveal the mechanisms of cell wall formation. In this study, we assembled de novo transcriptomes of three diatom species, Nitzschia palea , Achnanthes kuwaitensis , and Pseudoleyanella lunata , and compared protein-coding genes of five genome-sequenced diatom species. These analyses revealed a number of diatom-specific genes that encode putative endoplasmic reticulum-targeting proteins. Significant numbers of these proteins showed homology to silicanin-1, which is a conserved diatom protein that reportedly contributes to cell wall formation. These proteins also included a previously unrecognized SET domain protein methyltransferase family that may regulate functions of cell wall formation-related proteins and long-chain polyamines. Proteomic analysis of cell wall-associated proteins in N. palea identified a protein that is also encoded by one of the diatom-specific genes. Expression analysis showed that candidate genes were upregulated in response to silicon, suggesting that these genes play roles in silica cell wall formation. These candidate genes can facilitate further investigations of silica cell wall formation in diatoms.
Editor: New York: Springer US
Idioma: Inglês

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Comparative Gene Analysis Focused on Silica Cell Wall Formation: Identification of Diatom-Specific SET Domain Protein Methyltransferases

Nemoto, Michiko ; Iwaki, Sayako ; Moriya, Hisao ; Monden, Yuki ; Tamura, Takashi ; Inagaki, Kenji ; Mayama, Shigeki ; Obuse, Kiori

New York: Springer US

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