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The Microbiological Landscape of Anaerobic Infections in Hidradenitis Suppurativa: A Prospective Metagenomic Study

Guet-Revillet, Hélène ; Jais, Jean-Philippe ; Ungeheuer, Marie-Noelle ; Coignard-Biehler, Hélène ; Duchatelet, Sabine ; Delage, Maïa ; Lam, Thi ; Hovnanian, Alain ; Lortholary, Olivier ; Nassif, Xavier ; Nassif, Aude ; Join-Lambert, Olivier

Clinical infectious diseases, 2017-07, Vol.65 (2), p.282-291 [Periódico revisado por pares]

United States: Oxford University Press

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Título:
The Microbiological Landscape of Anaerobic Infections in Hidradenitis Suppurativa: A Prospective Metagenomic Study
Autor: Guet-Revillet, Hélène ; Jais, Jean-Philippe ; Ungeheuer, Marie-Noelle ; Coignard-Biehler, Hélène ; Duchatelet, Sabine ; Delage, Maïa ; Lam, Thi ; Hovnanian, Alain ; Lortholary, Olivier ; Nassif, Xavier ; Nassif, Aude ; Join-Lambert, Olivier
Assuntos: Abundance ; Actinomycetes ; Adult ; Anaerobes ; Anaerobic microorganisms ; Antiinfectives and antibacterials ; Antimicrobial agents ; ARTICLES AND COMMENTARIES ; Bacteria ; Bacteria, Anaerobic - genetics ; Bacteria, Anaerobic - isolation & purification ; Bacteria, Anaerobic - physiology ; Body mass ; Body mass index ; Commensals ; Construction ; Female ; Gene libraries ; Gene sequencing ; Genomics ; Gram-Negative Bacteria - genetics ; Gram-Negative Bacteria - isolation & purification ; Hidradenitis Suppurativa - microbiology ; Hidradenitis Suppurativa - physiopathology ; High-Throughput Nucleotide Sequencing - methods ; Humans ; Infections ; Inflammation ; Lesions ; Male ; Metagenomics ; Microbiology ; Microbiota ; Microorganisms ; Next-generation sequencing ; Paving ; Prevotella - isolation & purification ; Prospective Studies ; Quality of Life ; Remission ; Ribonucleic acid ; RNA ; rRNA 16S ; Skin - microbiology ; Skin - pathology ; Skin diseases ; Soft Tissue Infections - microbiology ; Taxa
É parte de: Clinical infectious diseases, 2017-07, Vol.65 (2), p.282-291
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Background. Hidradenitis suppurativa (HS) is a frequent and severe disease of the skin, characterized by recurrent or chronic skinfold suppurative lesions with a high impact on quality of life. Although considered inflammatory, antimicrobial treatments can improve or lead to clinical remission of HS, suggesting triggering microbial factors. Indeed, mixed anaerobic microbiota are associated with a majority of HS lesions. Our aim in this study was to characterize the landscape of anaerobic infections in HS using high-throughput sequencing. Methods. We sampled and cultured 149 lesions and 175 unaffected control skinfold areas from 65 adult HS patients. The microbiome of 80 anaerobic lesions was compared to that of 88 control samples by 454 high-throughput sequencing after construction of 16S ribosomal RNA gene libraries. Results. Bacterial cultures detected anaerobes in 83% of lesions vs 53% of control samples, combined with milleri group streptococci and actinomycetes in 33% and 26% of cases, respectively. High-throughput sequencing identified 43 taxa associated with HS lesions. Two gram-negative anaerobic rod taxa, Prevotella and Porphyromonas, predominated, contrasting with a reduced abundance of aerobic commensals. These rare taxa of normal skinfold microbiota were associated with lesions independently of gender, duration and familial history of HS, body mass index, and location. Two main additional taxa, Fusobacterium and Parvimonas, correlated with the clinical severity of HS. Conclusions. In this study we reveal the high prevalence and particular landscape of mixed anaerobic infection in HS, paving the way for rationale targeted antimicrobial treatments.
Editor: United States: Oxford University Press
Idioma: Inglês

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The Microbiological Landscape of Anaerobic Infections in Hidradenitis Suppurativa: A Prospective Metagenomic Study

Guet-Revillet, Hélène ; Jais, Jean-Philippe ; Ungeheuer, Marie-Noelle ; Coignard-Biehler, Hélène ; Duchatelet, Sabine ; Delage, Maïa ; Lam, Thi ; Hovnanian, Alain ; Lortholary, Olivier ; Nassif, Xavier ; Nassif, Aude ; Join-Lambert, Olivier

United States: Oxford University Press

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