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Depth-related distribution of a key gene of the tetraether lipid biosynthetic pathway in marine Thaumarchaeota

Villanueva, Laura ; Schouten, Stefan ; Sinninghe Damsté, Jaap S.

Environmental microbiology, 2015-10, Vol.17 (10), p.3527-3539 [Periódico revisado por pares]

England: Blackwell Publishing Ltd

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  • Título:
    Depth-related distribution of a key gene of the tetraether lipid biosynthetic pathway in marine Thaumarchaeota
  • Autor: Villanueva, Laura ; Schouten, Stefan ; Sinninghe Damsté, Jaap S.
  • Assuntos: Alkyl and Aryl Transferases - genetics ; Amino Acid Sequence ; Ammonia - metabolism ; Archaea - genetics ; Archaea - metabolism ; Biosynthetic Pathways - genetics ; Glycerol ; Glycerol - metabolism ; Lipids - analysis ; Lipids - biosynthesis ; Metagenomics ; Molecular Sequence Data ; Oxidoreductases - genetics ; Oxygen - analysis
  • É parte de: Environmental microbiology, 2015-10, Vol.17 (10), p.3527-3539
  • Notas: ark:/67375/WNG-P5TXRCB9-R
    ArticleID:EMI12508
    Fig. S1. Isoprenoid glycerol dialkyl glycerol tetraether (GDGT) structures.Fig. S2. Fractional abundance of GDGT-1, 2, 3, and crenarchaeol regioisomer in depth intervals in (A) Arabian Sea SPM, IPL-GDGT, (B) Arabian Sea SPM, CL-GDGT data, (C) Equatorial Pacific station 6, S1 (winter 1992), (D) Equatorial Pacific station 6, S2 (summer 1992) (Wuchter et al., 2005).Fig. S3. Abundance of archaeal and thaumarchaeotal 16S rRNA gene copies per litre in the Arabian Sea SPM as reported by Pitcher and colleagues (2011a) and Schouten and colleagues (2012).Fig. S4. GDGT-2/GDGT-3 ratio in the Arabian Sea and Equatorial Pacific SPM. CL indicates GDGT core lipids derived from dead material and IPL indicates intact polar lipid-GDGTs derived from living biomass.Table S1. Summary of physicochemical conditions and amoA gene quantification and gene expression in the Arabian Sea SPM and surface sediment reported in this study. Data were from Pitcher et al. (2011a), Kraal et al. (2012) and this study. Table S2. Metagenome projects from marine microbiomes in IMG/M database used in this study. Table S3. Metagenome projects from Camera database included in the 'deep water' database. Table S4. GGGP synthase gene primers designed and applied in this study.
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  • Descrição: Summary The distribution of isoprenoid glycerol dialkyl glycerol tetraethers (GDGT) lipids synthesized by Thaumarchaeota has been shown to be temperature‐dependent in world oceans. Depth‐related differences in the ammonia monooxygenase (amoA) of Thaumarchaeota have led to the classification of ‘shallow’ and ‘deep water’ clusters, potentially affecting GDGT distributions. Here, we investigate if this classification is also reflected in a key gene of the thaumarchaeotal lipid biosynthetic pathway coding for geranylgeranylglyceryl phosphate (GGGP) synthase. We investigated metagenomic databases, suspended particulate matter and surface sediment of the Arabian Sea oxygen minimum zone. These revealed significant differences in amoA and GGGP synthase between ‘shallow’ and ‘deep water’ Thaumarchaeota. Intriguingly, amoA and GGGP synthase sequences of benthic Thaumarchaeota clustered with the ‘shallow water’ rather than with ‘deep water’ Thaumarchaeota. This suggests that pressure and temperature are unlikely factors that drive the differentiation, and suggests an important role of ammonia concentration that is higher in benthic and ‘shallow water’ niches. Analysis of the relative abundance of GDGTs in the Arabian Sea and in globally distributed surface sediments showed differences in GDGT distributions from subsurface to deep waters that may be explained by differences in the GGGP synthase, suggesting a genetic control on GDGT distributions.
  • Editor: England: Blackwell Publishing Ltd
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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