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Vertical mother–neonate transfer of maternal gut bacteria via breastfeeding

Jost, Ted ; Lacroix, Christophe ; Braegger, Christian P ; Rochat, Florence ; Chassard, Christophe

Environmental microbiology, 2014-09, Vol.16 (9), p.2891-2904 [Periódico revisado por pares]

England: Blackwell Science

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Título:
Vertical mother–neonate transfer of maternal gut bacteria via breastfeeding
Autor: Jost, Ted ; Lacroix, Christophe ; Braegger, Christian P ; Rochat, Florence ; Chassard, Christophe
Assuntos: anaerobes ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria, Anaerobic - classification ; Bacteria, Anaerobic - genetics ; Bacteroides ; Bifidobacterium - classification ; Bifidobacterium - genetics ; Bifidobacterium breve ; Breast Feeding ; breast milk ; Breastfeeding & lactation ; Clostridium ; Collinsella ; Coprococcus ; digestive system ; DNA ; DNA Fingerprinting ; DNA, Bacterial - genetics ; ecosystems ; feces ; Feces - microbiology ; Female ; Gastrointestinal Tract - microbiology ; genes ; Gram-Positive Bacteria - classification ; Gram-Positive Bacteria - genetics ; Humans ; immune system ; Infant, Newborn ; intestinal microorganisms ; Milk ; Milk, Human - microbiology ; neonates ; polymerase chain reaction ; Probiotics ; pulsed-field gel electrophoresis ; ribosomal RNA ; RNA, Ribosomal, 16S - genetics ; Roseburia ; sequence analysis ; Sequence Analysis, DNA ; Veillonella
É parte de: Environmental microbiology, 2014-09, Vol.16 (9), p.2891-2904
Notas: http://dx.doi.org/10.1111/1462-2920.12238
Fig. S1. RAPD fingerprints of Bifidobacterium spp. isolated from maternal (MF) and neonatal faeces (NF) of mother-neonate pair N8, using primers (a) OPA-02, (b) OPL-07 and (c) OPL-16. Isolates highlighted in bold indicate identical Bifidobacterium longum strains isolated 10 days postpartum, which are shared between mother and neonate (isolates MF 4i7 and NF 4i2). Fig. S2. REP-PCR fingerprints of Lactobacillus spp. isolated from maternal faeces (MF), breast milk (BM) and neonatal faeces (NF) of mother-neonate pair N4, using primer (GTG)5. Isolates highlighted in bold indicate identical Lactobacillus casei strains isolated 12 days postpartum, which are shared between maternal and neonatal faeces (isolates MF 3i7 and NF 3i7). Table S1. Causes for exclusion of 14 out of 21 mothers-to-be/mother-neonate pairs from the observational clinical study.
ArticleID:EMI12238
Nestlé Nutrition (Vevey, Switzerland), Nestec (Lausanne, Switzerland)
Swiss Foundation for Nutrition Research (SFEFS) (Zurich, Switzerland)
istex:18BD5A53BBA206CF533EB4C744240045A162BD40
Nestec (Lausanne, Switzerland)
ark:/67375/WNG-K9K1TFSG-M
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Breast milk has recently been recognized as source of commensal and potential probiotic bacteria. The present study investigated whether viable strains of gut‐associated obligate anaerobes are shared between the maternal and neonatal gut ecosystem via breastfeeding. Maternal faeces, breast milk and corresponding neonatal faeces collected from seven mothers‐neonate pairs at three neonatal sampling points were analyzed by culture‐independent (pyrosequencing) and culture‐dependent methods (16S rRNA gene sequencing, pulsed field gel electrophoresis, random amplified polymorphic DNA and repetitive extragenic palindromic polymerase chain reaction. Pyrosequencing allowed identifying gut‐associated obligate anaerobic genera, like Bifidobacterium, Bacteroides, Parabacteroides and members of the Clostridia (Blautia, Clostridium, Collinsella and Veillonella) shared between maternal faeces, breast milk and neonatal faeces. Using culture, a viable strain of Bifidobacterium breve was shown to be shared between all three ecosystems within one mother–neonate pair. Furthermore, pyrosequencing revealed that several butyrate‐producing members of the Clostridia (Coprococcus, Faecalibacterium, Roseburia and Subdoligranulum) were shared between maternal faeces and breast milk. This study shows that (viable) obligate gut‐associated anaerobes may be vertically transferred from mother to neonate via breastfeeding. Thus, our data support the recently suggested hypothesis of a novel way of mother–neonate communication, in which maternal gut bacteria reach breast milk via an entero‐mammary pathway to influence neonatal gut colonization and maturation of the immune system.
Editor: England: Blackwell Science
Idioma: Inglês

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Vertical mother–neonate transfer of maternal gut bacteria via breastfeeding

Jost, Ted ; Lacroix, Christophe ; Braegger, Christian P ; Rochat, Florence ; Chassard, Christophe

England: Blackwell Science

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