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Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA

Murtaza, Muhammed ; Dawson, Sarah-Jane ; Tsui, Dana W Y ; Gale, Davina ; Forshew, Tim ; Piskorz, Anna M ; Parkinson, Christine ; Chin, Suet-Feung ; Kingsbury, Zoya ; Wong, Alvin S C ; Marass, Francesco ; Humphray, Sean ; Hadfield, James ; Bentley, David ; Chin, Tan Min ; Brenton, James D ; Caldas, Carlos ; Rosenfeld, Nitzan

Nature (London), 2013-05, Vol.497 (7447), p.108-112 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA
Autor: Murtaza, Muhammed ; Dawson, Sarah-Jane ; Tsui, Dana W Y ; Gale, Davina ; Forshew, Tim ; Piskorz, Anna M ; Parkinson, Christine ; Chin, Suet-Feung ; Kingsbury, Zoya ; Wong, Alvin S C ; Marass, Francesco ; Humphray, Sean ; Hadfield, James ; Bentley, David ; Chin, Tan Min ; Brenton, James D ; Caldas, Carlos ; Rosenfeld, Nitzan
Assuntos: Alleles ; Antimitotic agents ; Antineoplastic agents ; Antineoplastic Agents - pharmacology ; Antineoplastic Agents - therapeutic use ; Biopsy ; Blood plasma ; Breast cancer ; Breast Neoplasms - drug therapy ; Breast Neoplasms - genetics ; Breast Neoplasms - pathology ; Cancer ; Carcinoma, Non-Small-Cell Lung - drug therapy ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - pathology ; Care and treatment ; Class I Phosphatidylinositol 3-Kinases ; Deoxyribonucleic acid ; DNA ; DNA Mutational Analysis ; DNA sequencing ; DNA, Neoplasm - analysis ; DNA, Neoplasm - genetics ; Dosage and administration ; Drug resistance ; Drug Resistance, Neoplasm - drug effects ; Drug Resistance, Neoplasm - genetics ; ErbB Receptors - genetics ; Evolution, Molecular ; Exome - genetics ; Female ; Genetic aspects ; Genome, Human - genetics ; Genomes ; Genomics ; Humans ; Intercellular Signaling Peptides and Proteins - genetics ; Lung Neoplasms - drug therapy ; Lung Neoplasms - genetics ; Lung Neoplasms - pathology ; Mediator Complex Subunit 1 - genetics ; Mutation ; Neoplasms - drug therapy ; Neoplasms - genetics ; Neoplasms - pathology ; Nucleotide sequencing ; Ovarian Neoplasms - drug therapy ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - pathology ; Patients ; Phosphatidylinositol 3-Kinases - genetics ; Physiological aspects ; Plasma ; Plasma - chemistry ; Retinoblastoma Protein - genetics ; Tumors
É parte de: Nature (London), 2013-05, Vol.497 (7447), p.108-112
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SourceType-Scholarly Journals-1
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Descrição: Cancers acquire resistance to systemic treatment as a result of clonal evolution and selection. Repeat biopsies to study genomic evolution as a result of therapy are difficult, invasive and may be confounded by intra-tumour heterogeneity. Recent studies have shown that genomic alterations in solid cancers can be characterized by massively parallel sequencing of circulating cell-free tumour DNA released from cancer cells into plasma, representing a non-invasive liquid biopsy. Here we report sequencing of cancer exomes in serial plasma samples to track genomic evolution of metastatic cancers in response to therapy. Six patients with advanced breast, ovarian and lung cancers were followed over 1-2 years. For each case, exome sequencing was performed on 2-5 plasma samples (19 in total) spanning multiple courses of treatment, at selected time points when the allele fraction of tumour mutations in plasma was high, allowing improved sensitivity. For two cases, synchronous biopsies were also analysed, confirming genome-wide representation of the tumour genome in plasma. Quantification of allele fractions in plasma identified increased representation of mutant alleles in association with emergence of therapy resistance. These included an activating mutation in PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha) following treatment with paclitaxel; a truncating mutation in RB1 (retinoblastoma 1) following treatment with cisplatin; a truncating mutation in MED1 (mediator complex subunit 1) following treatment with tamoxifen and trastuzumab, and following subsequent treatment with lapatinib, a splicing mutation in GAS6 (growth arrest-specific 6) in the same patient; and a resistance-conferring mutation in EGFR (epidermal growth factor receptor; T790M) following treatment with gefitinib. These results establish proof of principle that exome-wide analysis of circulating tumour DNA could complement current invasive biopsy approaches to identify mutations associated with acquired drug resistance in advanced cancers. Serial analysis of cancer genomes in plasma constitutes a new paradigm for the study of clonal evolution in human cancers.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA

England: Nature Publishing Group

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