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On-fiber derivatization of spme extracts of phenol, hydroquinone and catechol with GC-MS detection

LOURENCO, E. L. B ; FERREIRA, A ; PINTO, E ; YONAMINE, M ; FARSKY, S. H. P

Chromatographia, 2006-02, Vol.63 (3-4), p.175-179 [Periódico revisado por pares]

Oxford: Springer

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Título:
On-fiber derivatization of spme extracts of phenol, hydroquinone and catechol with GC-MS detection
Autor: LOURENCO, E. L. B ; FERREIRA, A ; PINTO, E ; YONAMINE, M ; FARSKY, S. H. P
Assuntos: Acetic acid ; Acrylic resins ; Analytical chemistry ; Biological and medical sciences ; Catechol ; Chemical and industrial products toxicology. Toxic occupational diseases ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Coated fibers ; Coefficient of variation ; Deuteration ; Exact sciences and technology ; Gas chromatographic methods ; Gas chromatography ; Hydroquinone ; Mass spectrometry ; Medical sciences ; Metabolites ; Phenols ; Reagents ; Solid phases ; Toxicology ; Urine ; Vapor phases ; Various organic compounds
É parte de: Chromatographia, 2006-02, Vol.63 (3-4), p.175-179
Descrição: A gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of phenol (PHE), hydroquinone (HQ) and catechol (CAT) in urine was developed and validated. The method was based on the acidic hydrolysis of conjugated phenolic compounds and further extraction of analytes using solid-phase microextraction (SPME). Analytes were extracted by submersing the polar polyacrylate coated fiber (85 µm) into urine (adjusted to pH 3.0 with glacial acetic acid) for 20 min with magnetic stirring. The extracted compounds on the fiber were exposed to hexamethyldisilazane reagent in the vapor phase for 20 min to yield the corresponding trimethysililylated derivates. This on-fiber derivatization procedure allowed the formation of more amenable compounds for GC analysis, without adversely affecting the lifetime of the fiber. The MS was operated in the selected ion monitoring mode (SIM). The limits of detection were 0.3 µg mL−1 for PHE, 0.15 µg mL−1 for HQ and 0.02 µg mL−1 for CAT. Inter and intra-assay precisions were also verified (coefficient of variation < 8%) with the use of deuterated internal standards. This method of GC-MS analysis can be readily utilized to monitor PHE and its metabolites (HQ and CAT) in urine samples.
Editor: Oxford: Springer
Idioma: Inglês

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On-fiber derivatization of spme extracts of phenol, hydroquinone and catechol with GC-MS detection

LOURENCO, E. L. B ; FERREIRA, A ; PINTO, E ; YONAMINE, M ; FARSKY, S. H. P

Oxford: Springer

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