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Exploring the expression and potential function of follicle stimulating hormone receptor in extragonadal cells related to abdominal aortic aneurysm

Tedjawirja, V N ; Mieremet, A ; Rombouts, K B ; Yap, C ; Neele, A E ; Northoff, B H ; Chen, H J ; Vos, M ; Klaver, D ; Yeung, K K ; Balm, R ; de Waard, V

PloS one, 2023-05, Vol.18 (5), p.e0285607-e0285607 [Periódico revisado por pares]

United States: Public Library of Science

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Exploring the expression and potential function of follicle stimulating hormone receptor in extragonadal cells related to abdominal aortic aneurysm
Autor: Tedjawirja, V N ; Mieremet, A ; Rombouts, K B ; Yap, C ; Neele, A E ; Northoff, B H ; Chen, H J ; Vos, M ; Klaver, D ; Yeung, K K ; Balm, R ; de Waard, V
Assuntos: Abdomen ; Abdominal aneurysm ; Aged ; Analysis ; Aorta ; Aortic Aneurysm, Abdominal - genetics ; Aortic aneurysms ; Biology and Life Sciences ; Cell activation ; Cell differentiation ; Cell surface ; Coronary vessels ; Datasets ; Diagnosis ; Endothelial cells ; Endothelial Cells - metabolism ; Estrogens ; Experiments ; Female ; Flow cytometry ; Follicle Stimulating Hormone - metabolism ; Follicle Stimulating Hormone - pharmacology ; Follicle Stimulating Hormone, Human ; Follicle-stimulating hormone ; Follicles ; Gene expression ; Genes ; Genetic aspects ; Glycoproteins ; Health aspects ; Hormone receptors ; Humans ; Inflammation ; Low density lipoprotein ; Low density lipoproteins ; Low level ; Macrophages ; Male ; Medicine and Health Sciences ; Monocytes ; Muscles ; Post-menopause ; Postmenopausal women ; Proteins ; R&D ; Receptors, FSH - genetics ; Research & development ; Research and Analysis Methods ; Scientific equipment and supplies industry ; Smooth muscle ; Stimulation ; Stimuli ; Testis - metabolism ; Tissues ; Umbilical cord ; Veins & arteries
É parte de: PloS one, 2023-05, Vol.18 (5), p.e0285607-e0285607
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Competing Interests: The authors have declared that no competing interests exist.
Descrição: Follicle stimulating hormone (FSH) is identified to play a role in postmenopausal disease and hypothesized to affect abdominal aortic aneurysm (AAA) onset/progression in postmenopausal women. We aimed to detect FSHR gene expression in AAA tissue and cell types involved in AAA formation. FSH stimulation of human umbilical cord endothelial cells (HUVECs), smooth muscle cells (HUCs) and PMA-differentiated macrophages to assess gene expression of FSHR and various markers. Human macrophages activated with various stimuli were assessed for FSHR gene expression. AAA dataset, AAA tissue samples and AAA-derived smooth muscle cells (SMC) obtained from elderly female donors were assessed for FSHR gene expression. AAA-SMCs were stimulated with FSH to assess its effect on gene expression. Lastly, oxidized low-density-lipoprotein (ox-LDL) uptake and abundance of cell surface protein markers were assessed by flow cytometry after FSH stimulation of human monocytes. FSH stimulation showed similar levels of gene expression in HUVECs and HUCs. Only ACTA2 was downregulated in HUCs. In PMA-differentiated macrophages, gene expression of inflammation markers was unchanged after FSH stimulation. FSHR gene expression was found to be low in the AAA datasets. Female AAA-SMCs show occasional FSHR gene expression at a very low level, yet stimulation with FSH did not affect gene expression of SMC- or inflammation markers. FSH stimulation did not impact ox-LDL uptake or alter cell surface protein expression in monocytes. While FSHR gene expression was detected in human testis tissue, it was below quantification level in all other investigated cell types, even upon activation of macrophages with various stimuli. Despite previous reports, we did not detect FSHR gene expression in various extragonadal cell types, except in occasional female AAA-SMCs. No clear effect on cell activation was observed upon FSH stimulation in any cell type. Our data suggest that a direct effect of FSH in AAA-related extragonadal cells is unlikely to influence AAA.
Editor: United States: Public Library of Science
Idioma: Inglês

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Exploring the expression and potential function of follicle stimulating hormone receptor in extragonadal cells related to abdominal aortic aneurysm

Tedjawirja, V N ; Mieremet, A ; Rombouts, K B ; Yap, C ; Neele, A E ; Northoff, B H ; Chen, H J ; Vos, M ; Klaver, D ; Yeung, K K ; Balm, R ; de Waard, V

United States: Public Library of Science

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