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3,3'-Diindolylmethane induces apoptosis and autophagy in fission yeast

Emami, Parvaneh ; Ueno, Masaru Sugiura, Reiko

PloS one, 2021-12, Vol.16 (12), p.e0255758 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
3,3'-Diindolylmethane induces apoptosis and autophagy in fission yeast
Autor: Emami, Parvaneh ; Ueno, Masaru
Sugiura, Reiko
Assuntos: Analysis ; Apoptosis ; Apoptosis - drug effects ; Apoptosis - genetics ; Autophagy ; Autophagy (Cytology) ; Autophagy - drug effects ; Autophagy - genetics ; Biology and Life Sciences ; Breast cancer ; Broccoli ; Cancer therapies ; Cell death ; Cell survival ; Chromosomes ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Dose-Response Relationship, Drug ; Indole-3-carbinol ; Indoles - pharmacology ; Life span ; Medicine and Health Sciences ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Methanol ; Mutants ; Mutation ; Nitrogen ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Observations ; Phagocytosis ; Physical Sciences ; Research and Analysis Methods ; Schizosaccharomyces - genetics ; Schizosaccharomyces - metabolism ; Schizosaccharomyces pombe Proteins - genetics ; Schizosaccharomyces pombe Proteins - metabolism ; Testing ; Yeast ; Yeasts
É parte de: PloS one, 2021-12, Vol.16 (12), p.e0255758
Notas: Competing Interests: The authors have declared that no competing interests exist.
Descrição: 3,3'-Diindolylmethane (DIM) is a compound derived from the digestion of indole-3-carbinol, found in the broccoli family. It induces apoptosis and autophagy in some types of human cancer. DIM extends lifespan in the fission yeast Schizosaccharomyces pombe. The mechanisms by which DIM induces apoptosis and autophagy in humans and expands lifespan in fission yeasts are not fully understood. Here, we show that DIM induces apoptosis and autophagy in log-phase cells, which is dose-dependent in fission yeast. A high concentration of DIM disrupted the nuclear envelope (NE) structure and induced chromosome condensation at an early time point. In contrast, a low concentration of DIM induced autophagy but did not disrupt NE structure. The mutant defective in autophagy was more sensitive to a low concentration of DIM, demonstrating that the autophagic pathway contributes to the survival of cells against DIM. Moreover, our results showed that the lem2 mutant is more sensitive to DIM. NE in the lem2 mutant was disrupted even at the low concentration of DIM. Our results demonstrate that the autophagic pathway and NE integrity are important to maintain viability in the presence of a low concentration of DIM. The mechanism of apoptosis and autophagy induction by DIM might be conserved in fission yeast and humans. Further studies will contribute to the understanding of the mechanism of apoptosis and autophagy by DIM in fission yeast and humans.
Editor: United States: Public Library of Science
Idioma: Inglês

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3,3'-Diindolylmethane induces apoptosis and autophagy in fission yeast

Emami, Parvaneh ; Ueno, Masaru Sugiura, Reiko

United States: Public Library of Science

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