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Improved islet recovery and efficacy through co-culture and co-transplantation of islets with human adipose-derived mesenchymal stem cells

Gamble, Anissa ; Pawlick, Rena ; Pepper, Andrew R ; Bruni, Antonio ; Adesida, Adetola ; Senior, Peter A ; Korbutt, Gregory S ; Shapiro, A M James Stepkowski, Stanislaw

PloS one, 2018-11, Vol.13 (11), p.e0206449-e0206449 [Periódico revisado por pares]

United States: Public Library of Science

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Improved islet recovery and efficacy through co-culture and co-transplantation of islets with human adipose-derived mesenchymal stem cells
Autor: Gamble, Anissa ; Pawlick, Rena ; Pepper, Andrew R ; Bruni, Antonio ; Adesida, Adetola ; Senior, Peter A ; Korbutt, Gregory S ; Shapiro, A M James
Stepkowski, Stanislaw
Assuntos: Adipose Tissue - cytology ; Allografts ; Angiogenesis ; Animals ; Anti-inflammatory agents ; Antiinflammatory agents ; Apoptosis ; Beta cells ; Biology and Life Sciences ; Bone marrow ; Cell culture ; Cell death ; Coculture Techniques ; Control ; Cytokines ; Diabetes ; Diabetes mellitus ; Diabetes mellitus (insulin dependent) ; Engraftment ; Female ; Fibroblasts ; Gene expression ; Grafts ; Growth factors ; Humans ; Hypoglycemia ; Hypoxia ; Inflammation ; Insulin ; Insulin secretion ; Islet cell transplantation ; Islet cells ; Islets of Langerhans - cytology ; Islets of Langerhans Transplantation - methods ; Kidney - cytology ; Laboratories ; Male ; Medicine and Health Sciences ; Mesenchymal Stem Cell Transplantation ; Mesenchymal Stem Cells - cytology ; Mesenchyme ; Mice ; Mice, Inbred BALB C ; Pancreatic islet transplantation ; Patient outcomes ; Recovery (Medical) ; Rodents ; Secretion ; Stem cell transplantation ; Stem cells ; Surgery ; Syngeneic grafts ; Systematic review ; Time Factors ; Transplantation ; Transplants & implants ; Type 1 diabetes ; Viability
É parte de: PloS one, 2018-11, Vol.13 (11), p.e0206449-e0206449
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Current address: Laboratory of Stem Cell Biology and Orthopaedic Tissue Engineering. University of Alberta, Edmonton, AB, Canada
These authors are co-senior authors on this work.
Descrição: Islet transplantation is an established clinical procedure for select patients with type 1 diabetes and severe hypoglycemia to stabilize glycemic control. Post-transplant, substantial beta cell mass is lost, necessitating multiple donors to maintain euglycemia. A potential strategy to augment islet engraftment is the co-transplantation of islets with multipotent mesenchymal stem cells to capitalize upon their pro-angiogenic and anti-inflammatory properties. Herein, we examine the in vitro and in vivo effect of co-culturing murine islets with human adipose-derived mesenchymal stem cells (Ad-MSCs). Islets co-cultured with Ad-MSCs for 48 hours had decreased cell death, superior viability as measured by membrane integrity, improved glucose stimulated insulin secretion and reduced apoptosis compared to control islets. These observations were recapitulated with human islets, albeit tested in a limited capacity. Recipients of marginal mouse islet mass grafts, co-transplanted with Ad-MSCs without a co-culture period, did not reverse to normoglycemia as efficiently as islets alone. However, utilizing a 48-hour co-culture period, marginal mouse islets grafts with Ad-MSCs achieved a superior percent euglycemia rate when compared to islets cultured and transplanted alone. A co-culture period of human islets with human Ad-MSCs may have a clinical benefit improving engraftment outcomes.
Editor: United States: Public Library of Science
Idioma: Inglês

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Improved islet recovery and efficacy through co-culture and co-transplantation of islets with human adipose-derived mesenchymal stem cells

Gamble, Anissa ; Pawlick, Rena ; Pepper, Andrew R ; Bruni, Antonio ; Adesida, Adetola ; Senior, Peter A ; Korbutt, Gregory S ; Shapiro, A M James Stepkowski, Stanislaw

United States: Public Library of Science

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