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Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections

Gomes-Fernandes, Meissiner ; Laabei, Maisem ; Pagan, Natalia ; Hidalgo, Jessica ; Molinos, Sònia ; Villar Hernandez, Raquel ; Domínguez-Villanueva, Dídac ; Jenkins, A Toby A ; Lacoma, Alicia ; Prat, Cristina Kluytmans, Jan

PloS one, 2017-04, Vol.12 (4), p.e0175552-e0175552 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections
Autor: Gomes-Fernandes, Meissiner ; Laabei, Maisem ; Pagan, Natalia ; Hidalgo, Jessica ; Molinos, Sònia ; Villar Hernandez, Raquel ; Domínguez-Villanueva, Dídac ; Jenkins, A Toby A ; Lacoma, Alicia ; Prat, Cristina
Kluytmans, Jan
Assuntos: Assaying ; Bacteremia ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biology ; Biology and Life Sciences ; Causes of ; Clinical isolates ; Colonization ; Complications ; Cyclic AMP ; Deoxyribonucleic acid ; DNA ; DNA microarrays ; DNA, Bacterial - genetics ; DNA, Bacterial - metabolism ; Gene expression ; Genes, Regulator - genetics ; Genetic aspects ; Genetic variation ; Genotype ; Hemolysin Proteins - genetics ; Hemolysin Proteins - metabolism ; Hospitals ; Humans ; Infections ; Infectious diseases ; Lysis ; Medicine and Health Sciences ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Pathogens ; Phenols ; Physiological aspects ; Polymerase chain reaction ; Real-Time Polymerase Chain Reaction ; Research and analysis methods ; Respiratory tract ; Respiratory tract diseases ; Respiratory tract infection ; Respiratory tract infections ; Respiratory Tract Infections - microbiology ; Respiratory Tract Infections - pathology ; Signal transduction ; Staphylococcus aureus ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Staphylococcus infections ; Statistical analysis ; Strains (organisms)
É parte de: PloS one, 2017-04, Vol.12 (4), p.e0175552-e0175552
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
These authors also contributed equally to this work and are co-senior authors on this work.
Conceptualization: CP AL.Data curation: MGF ML AL.Formal analysis: MGF ML AL.Funding acquisition: CP AL.Investigation: MGF ML AL.Methodology: ATAJ ML MGF JH NP DDV RVH.Project administration: CP AL.Resources: SM ATAJ.Supervision: CP AL.Validation: MGF ML CP AL.Visualization: MGF ML.Writing – original draft: MGF ML.Writing – review & editing: ML CP AL.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Characterization of Staphylococcus aureus clinical isolates derived from lower respiratory tract infections (LRTIs), and correlation between the functionality of the accessory gene regulator (Agr) and genotypic and phenotypic characteristics, clinical variables and clinical outcome. S aureus isolates derived from LRTIs and control groups (nasal carriage and bacteraemia) were genotyped using StaphyType DNA microarray. Agr activity was evaluated using the CAMP synergistic haemolysis assay and the Vesicle Lysis Test (VLT). Discordant strains were analysed by quantitative reverse-transcriptase real-time PCR (qRT-PCR). Agr was functional in 79.7% and 84.5% of strains according to the CAMP and VLT assays respectively. Higher concordance with RNAIII expression measured by qRT-PCR was observed with the VLT assay (76.2%) compared with the CAMP assay (23.8%). No statistically significant differences were observed in Agr functionality between the study groups, nor the phenotypical/genotypical bacterial characteristics. No association between increased mortality/respiratory complications and Agr function was observed. Agr activity was high (82.2%) in isolates from LRTIs suggesting the importance of this global regulator in lower respiratory tract colonisation and infection. However, equally high Agr activity was observed in isolates derived from nasal carriage and bacteraemia, contradictory to previous observations. Agr functionality measured by the VLT assay was superior to CAMP assay.
Editor: United States: Public Library of Science
Idioma: Inglês

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Accessory gene regulator (Agr) functionality in Staphylococcus aureus derived from lower respiratory tract infections

Gomes-Fernandes, Meissiner ; Laabei, Maisem ; Pagan, Natalia ; Hidalgo, Jessica ; Molinos, Sònia ; Villar Hernandez, Raquel ; Domínguez-Villanueva, Dídac ; Jenkins, A Toby A ; Lacoma, Alicia ; Prat, Cristina Kluytmans, Jan

United States: Public Library of Science

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