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A Comparison of Three Quantitative Methods to Estimate G6PD Activity in the Chittagong Hill Tracts, Bangladesh

Ley, Benedikt ; Alam, Mohammad Shafiul ; O'Donnell, James J ; Hossain, Mohammad Sharif ; Kibria, Mohammad Golam ; Jahan, Nusrat ; Khan, Wasif A ; Thriemer, Kamala ; Chatfield, Mark D ; Price, Ric N ; Richards, Jack S Langsley, Gordon

PloS one, 2017-01, Vol.12 (1), p.e0169930-e0169930 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
A Comparison of Three Quantitative Methods to Estimate G6PD Activity in the Chittagong Hill Tracts, Bangladesh
Autor: Ley, Benedikt ; Alam, Mohammad Shafiul ; O'Donnell, James J ; Hossain, Mohammad Sharif ; Kibria, Mohammad Golam ; Jahan, Nusrat ; Khan, Wasif A ; Thriemer, Kamala ; Chatfield, Mark D ; Price, Ric N ; Richards, Jack S
Langsley, Gordon
Assuntos: Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bangladesh - epidemiology ; Biology and Life Sciences ; Biomedical research ; Biosensing Techniques - instrumentation ; Biosensors ; Child ; Child, Preschool ; Colorimetry - instrumentation ; Confidence intervals ; Cross-Sectional Studies ; Dehydrogenases ; Diagnostic tests ; Dosage and administration ; Drug therapy ; Endemic Diseases ; Engineering and Technology ; Enzymes ; Female ; Glucose ; Glucose 6 phosphate dehydrogenase ; Glucosephosphate dehydrogenase ; Glucosephosphate Dehydrogenase - blood ; Glucosephosphate Dehydrogenase Deficiency - blood ; Glucosephosphate Dehydrogenase Deficiency - epidemiology ; Glucosephosphate Dehydrogenase Deficiency - ethnology ; Hemoglobin ; Hemoglobins - analysis ; Hemolysis ; Hemolysis and hemolysins ; Histology ; Humans ; Hygiene ; Infectious diseases ; Laboratories ; Malaria ; Malaria - epidemiology ; Male ; Medical research ; Medicine ; Medicine and Health Sciences ; Middle Aged ; Oxidative stress ; Phosphates ; Point-of-Care Systems ; Predictive Value of Tests ; Primaquine ; Research and Analysis Methods ; Risk factors ; ROC Curve ; Sensitivity and Specificity ; Spectrophotometry ; Spectrophotometry, Ultraviolet - instrumentation ; Young Adult
É parte de: PloS one, 2017-01, Vol.12 (1), p.e0169930-e0169930
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Competing Interests: JSR and JJO have a provisional patent on the WST-8 assay. This patent titled "Screening assay" was filed with IP Australia (application number 2016900333) on the 3 February 2016. They hold no other conflicts of interest related to employment, consultancies, or other products related to this work. This does not alter our adherence to PLOS ONE policies on sharing data and materials. We declare that there is no other conflict of interest for all other authors.
Conceptualization: BL RNP.Data curation: BL MSH.Formal analysis: BL MDC JSR RNP.Funding acquisition: BL RNP.Investigation: JJOD MGK NJ.Methodology: BL JSR RNP.Project administration: BL MSA.Resources: WAK JSR.Software: MSH.Supervision: BL JJOD WAK RNP.Validation: BL JJOD MSH KT JSR RNP.Visualization: BL.Writing – original draft: BL.Writing – review & editing: BL MSA WAK KT MDC JSR RNP.
Descrição: Glucose-6-phosphate-dehydrogenase-deficiency (G6PDd) is a major risk factor for primaquine-induced haemolysis. There is a need for improved point-of-care and laboratory-based G6PD diagnostics to unsure safe use of primaquine. G6PD activities of participants in a cross-sectional survey in Bangladesh were assessed using two novel quantitative assays, the modified WST-8 test and the CareStart™ G6PD Biosensor (Access Bio), The results were compared with a gold standard UV spectrophotometry assay (Randox). The handheld CareStart™ Hb instrument (Access Bio) is designed to be a companion instrument to the CareStart™ G6PD biosensor, and its performance was compared to the well-validated HemoCue™ method. All quantitative G6PD results were normalized with the HemoCue™ result. A total of 1002 individuals were enrolled. The adjusted male median (AMM) derived by spectrophotometry was 7.03 U/g Hb (interquartile range (IQR): 5.38-8.69), by WST-8 was 7.03 U/g Hb (IQR: 5.22-8.16) and by Biosensor was 8.61 U/g Hb (IQR: 6.71-10.08). The AMM between spectrophotometry and WST-8 did not differ (p = 1.0) but differed significantly between spectrophotometry and Biosensor (p<0.01). Both, WST-8 and Biosensor were correlated with spectrophotometry (rs = 0.5 and rs = 0.4, both p<0.001). The mean difference in G6PD activity was -0.12 U/g Hb (95% limit of agreement (95% LoA): -5.45 to 5.20) between spectrophotometry and WST-8 and -1.74U/g Hb (95% LoA: -7.63 to 4.23) between spectrophotometry and Biosensor. The WST-8 identified 55.1% (49/89) and the Biosensor 19.1% (17/89) of individuals with G6PD activity <30% by spectrophotometry. Areas under the ROC curve did not differ significantly for the WST-8 and Biosensor irrespective of the cut-off activity applied (all p>0.05). Sensitivity and specificity for detecting G6PD activity <30% was 0.55 (95% confidence interval (95%CI): 0.44-0.66) and 0.98 (95%CI: 0.97-0.99) respectively for the WST-8 and 0.19 (95%CI: 0.12-0.29) and 0.99 (95%CI: 0.98-0.99) respectively for the Biosensor. Hb concentrations measured by HemoCue™ and CareStart™ Hb were strongly correlated (rs = 0.8, p<0.001, mean difference = 0.09 g Hb/dL, 95% LoA: -2.15 to 2.34). WST-8 and the CareStart™ G6PD Biosensor represent advances in G6PD diagnostics in resource poor settings, but will require further development before clinical deployment. The CareStart™ Hb instrument produced a precise measure of haemoglobin concentration.
Editor: United States: Public Library of Science
Idioma: Inglês

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A Comparison of Three Quantitative Methods to Estimate G6PD Activity in the Chittagong Hill Tracts, Bangladesh

Ley, Benedikt ; Alam, Mohammad Shafiul ; O'Donnell, James J ; Hossain, Mohammad Sharif ; Kibria, Mohammad Golam ; Jahan, Nusrat ; Khan, Wasif A ; Thriemer, Kamala ; Chatfield, Mark D ; Price, Ric N ; Richards, Jack S Langsley, Gordon

United States: Public Library of Science

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