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Pandemic H1N1 influenza isolated from free-ranging Northern Elephant Seals in 2010 off the central California coast

Goldstein, Tracey ; Mena, Ignacio ; Anthony, Simon J ; Medina, Rafael ; Robinson, Patrick W ; Greig, Denise J ; Costa, Daniel P ; Lipkin, W Ian ; Garcia-Sastre, Adolfo ; Boyce, Walter M Davis, Ian C.

PloS one, 2013-05, Vol.8 (5), p.e62259-e62259 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Pandemic H1N1 influenza isolated from free-ranging Northern Elephant Seals in 2010 off the central California coast
Autor: Goldstein, Tracey ; Mena, Ignacio ; Anthony, Simon J ; Medina, Rafael ; Robinson, Patrick W ; Greig, Denise J ; Costa, Daniel P ; Lipkin, W Ian ; Garcia-Sastre, Adolfo ; Boyce, Walter M
Davis, Ian C.
Assuntos: Analysis ; Animal behavior ; Animals ; Antibodies ; Aquatic birds ; Behavior ; Biology ; Birds ; Breeding ; California ; Cell cycle ; Cells, Cultured ; Disease ; Disease transmission ; DNA sequencing ; Dogs ; Earth Sciences ; Ecological effects ; Ecology ; Eggs ; Embryos ; Exposure ; Female ; Females ; Gene sequencing ; Genomes ; Genomics ; Health surveillance ; Homology ; Humans ; Immunology ; Infections ; Influenza ; Influenza A ; Influenza A Virus, H1N1 Subtype - isolation & purification ; Influenza virus ; Influenza viruses ; Inoculation ; Interspecific ; Kidney transplantation ; Laboratories ; Livestock ; Marine ; Marine mammals ; Medicine ; Mirounga angustirostris ; Nucleotide sequencing ; Orthomyxoviridae ; Orthomyxoviridae Infections - epidemiology ; Orthomyxoviridae Infections - virology ; Pandemics ; Pneumonia ; Polymerase chain reaction ; Public health ; Replication ; Seals ; Seals, Earless ; Surveillance ; Swine ; Swine flu ; Swine influenza ; Syngeneic grafts ; Veterinary colleges ; Veterinary medicine ; Veterinary Science ; Viruses ; Wildlife
É parte de: PloS one, 2013-05, Vol.8 (5), p.e62259-e62259
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: TG WMB IM AG-S RM. Performed the experiments: TG IM SJA PWR DJG. Analyzed the data: TG IM SJA RM PWR WMB AG-S. Contributed reagents/materials/analysis tools: WMB DPC WIL AG-S DJG. Wrote the paper: TG IM RM PWR AG-S WMB.
Descrição: Interspecies transmission of influenza A is an important factor in the evolution and ecology of influenza viruses. Marine mammals are in contact with a number of influenza reservoirs, including aquatic birds and humans, and this may facilitate transmission among avian and mammalian hosts. Virus isolation, whole genome sequencing, and hemagluttination inhibition assay confirmed that exposure to pandemic H1N1 influenza virus occurred among free-ranging Northern Elephant Seals (Mirounga angustirostris) in 2010. Nasal swabs were collected from 42 adult female seals in April 2010, just after the animals had returned to the central California coast from their short post-breeding migration in the northeast Pacific. Swabs from two seals tested positive by RT-PCR for the matrix gene, and virus was isolated from each by inoculation into embryonic chicken eggs. Whole genome sequencing revealed greater than 99% homology with A/California/04/2009 (H1N1) that emerged in humans from swine in 2009. Analysis of more than 300 serum samples showed that samples collected early in 2010 (n = 100) were negative and by April animals began to test positive for antibodies against the pH1N1 virus (HI titer of ≥1∶40), supporting the molecular findings. In vitro characterizations studies revealed that viral replication was indistinguishable from that of reference strains of pH1N1 in canine kidney cells, but replication was inefficient in human epithelial respiratory cells, indicating these isolates may be elephant seal adapted viruses. Thus findings confirmed that exposure to pandemic H1N1 that was circulating in people in 2009 occurred among free-ranging Northern Elephant Seals in 2010 off the central California coast. This is the first report of pH1N1 (A/Elephant seal/California/1/2010) in any marine mammal and provides evidence for cross species transmission of influenza viruses in free-ranging wildlife and movement of influenza viruses between humans and wildlife.
Editor: United States: Public Library of Science
Idioma: Inglês

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Pandemic H1N1 influenza isolated from free-ranging Northern Elephant Seals in 2010 off the central California coast

Goldstein, Tracey ; Mena, Ignacio ; Anthony, Simon J ; Medina, Rafael ; Robinson, Patrick W ; Greig, Denise J ; Costa, Daniel P ; Lipkin, W Ian ; Garcia-Sastre, Adolfo ; Boyce, Walter M Davis, Ian C.

United States: Public Library of Science

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