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Assessing the effects of Aedes aegypti kdr mutations on pyrethroid resistance and its fitness cost

Brito, Luiz Paulo ; Linss, Jutta G B ; Lima-Camara, Tamara N ; Belinato, Thiago A ; Peixoto, Alexandre A ; Lima, José Bento P ; Valle, Denise ; Martins, Ademir J Kumar, Nirbhay

PloS one, 2013-04, Vol.8 (4), p.e60878 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Assessing the effects of Aedes aegypti kdr mutations on pyrethroid resistance and its fitness cost
Autor: Brito, Luiz Paulo ; Linss, Jutta G B ; Lima-Camara, Tamara N ; Belinato, Thiago A ; Peixoto, Alexandre A ; Lima, José Bento P ; Valle, Denise ; Martins, Ademir J
Kumar, Nirbhay
Assuntos: Adults ; Aedes - drug effects ; Aedes - genetics ; Aedes - growth & development ; Aedes - physiology ; Aedes aegypti ; Agriculture ; Alleles ; Analysis ; Animal Feed ; Animals ; Aquatic insects ; Biology ; Circadian Rhythm - drug effects ; Circadian Rhythm - genetics ; Culex pipiens ; Culicidae ; Dengue ; Dengue fever ; Diptera ; Disease ; Drug Resistance - genetics ; Economic aspects ; Eggs ; Enzymes ; Female ; Females ; Fertility - drug effects ; Fertility - genetics ; Fitness ; Gene Frequency ; Gene mutation ; Gene mutations ; Genetic aspects ; Homozygote ; Insecticide resistance ; Insecticides ; Insemination - drug effects ; Insemination - genetics ; Laboratories ; Larva - drug effects ; Larva - growth & development ; Larvae ; Larval development ; Locomotor activity ; Longevity - drug effects ; Longevity - genetics ; Male ; Medicine ; Mosquitoes ; Motor Activity - drug effects ; Motor Activity - genetics ; Mutation ; Ovum - drug effects ; Parents ; Population genetics ; Pupa - drug effects ; Pupa - growth & development ; Pyrethrins ; Pyrethroids ; Reproductive fitness ; Sodium ; Sodium channels (voltage-gated) ; Tropical diseases ; Vector-borne diseases ; Voltage-Gated Sodium Channels - genetics
É parte de: PloS one, 2013-04, Vol.8 (4), p.e60878
Notas: Conceived and designed the experiments: AJM JBPL AAP DV. Performed the experiments: LPB JGBL AJM TAB TNLC. Analyzed the data: LPB AJM AAP DV. Contributed reagents/materials/analysis tools: AAP DV. Wrote the paper: AJM DV AAP.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: Pyrethroids are the most used insecticide class worldwide. They target the voltage gated sodium channel (NaV), inducing the knockdown effect. In Aedes aegypti, the main dengue vector, the AaNaV substitutions Val1016Ile and Phe1534Cys are the most important knockdown resistance (kdr) mutations. We evaluated the fitness cost of these kdr mutations related to distinct aspects of development and reproduction, in the absence of any other major resistance mechanism. To accomplish this, we initially set up 68 crosses with mosquitoes from a natural population. Allele-specific PCR revealed that one couple, the one originating the CIT-32 strain, had both parents homozygous for both kdr mutations. However, this pyrethroid resistant strain also presented high levels of detoxifying enzymes, which synergistically account for resistance, as revealed by biological and biochemical assays. Therefore, we carried out backcrosses between CIT-32 and Rockefeller (an insecticide susceptible strain) for eight generations in order to bring the kdr mutation into a susceptible genetic background. This new strain, named Rock-kdr, was highly resistant to pyrethroid and presented reduced alteration of detoxifying activity. Fitness of the Rock-kdr was then evaluated in comparison with Rockefeller. In this strain, larval development took longer, adults had an increased locomotor activity, fewer females laid eggs, and produced a lower number of eggs. Under an inter-strain competition scenario, the Rock-kdr larvae developed even slower. Moreover, when Rockefeller and Rock-kdr were reared together in population cage experiments during 15 generations in absence of insecticide, the mutant allele decreased in frequency. These results strongly suggest that the Ae. aegypti kdr mutations have a high fitness cost. Therefore, enhanced surveillance for resistance should be priority in localities where the kdr mutation is found before new adaptive alleles can be selected for diminishing the kdr deleterious effects.
Editor: United States: Public Library of Science
Idioma: Inglês

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Assessing the effects of Aedes aegypti kdr mutations on pyrethroid resistance and its fitness cost

Brito, Luiz Paulo ; Linss, Jutta G B ; Lima-Camara, Tamara N ; Belinato, Thiago A ; Peixoto, Alexandre A ; Lima, José Bento P ; Valle, Denise ; Martins, Ademir J Kumar, Nirbhay

United States: Public Library of Science

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