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Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts

Bertram, Stephanie ; Heurich, Adeline ; Lavender, Hayley ; Gierer, Stefanie ; Danisch, Simon ; Perin, Paula ; Lucas, Jared M ; Nelson, Peter S ; Pöhlmann, Stefan ; Soilleux, Elizabeth J Thiel, Volker

PloS one, 2012-04, Vol.7 (4), p.e35876-e35876 [Periódico revisado por pares]

United States: Public Library of Science

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Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts
Autor: Bertram, Stephanie ; Heurich, Adeline ; Lavender, Hayley ; Gierer, Stefanie ; Danisch, Simon ; Perin, Paula ; Lucas, Jared M ; Nelson, Peter S ; Pöhlmann, Stefan ; Soilleux, Elizabeth J
Thiel, Volker
Assuntos: Amino acids ; Angiotensin-Converting Enzyme 2 ; Animals ; Binding sites ; Biology ; Birds ; Cell culture ; Cell Line ; Coronaviridae ; Coronaviruses ; Disease Reservoirs - veterinary ; Disease Reservoirs - virology ; Enzyme Activation ; Enzymes ; Epiglottis ; Gastrointestinal system ; Gastrointestinal Tract - enzymology ; Gastrointestinal Tract - virology ; Gene Expression ; Human tissues ; Humans ; Infections ; Influenza ; Influenza viruses ; Influenza, Human - enzymology ; Influenza, Human - genetics ; Influenza, Human - transmission ; Influenza, Human - virology ; Laboratories ; Medical research ; Medicine ; Orthomyxoviridae - physiology ; Pandemics ; Pathogenesis ; Peptidyl-Dipeptidase A - genetics ; Peptidyl-Dipeptidase A - metabolism ; Plasmids ; Proteases ; Proteins ; Receptors, Virus - genetics ; Receptors, Virus - metabolism ; Respiratory System - enzymology ; Respiratory System - virology ; Respiratory tract ; Serine ; Serine Endopeptidases - genetics ; Serine Endopeptidases - metabolism ; Severe acute respiratory syndrome ; Severe Acute Respiratory Syndrome - enzymology ; Severe Acute Respiratory Syndrome - genetics ; Severe Acute Respiratory Syndrome - transmission ; Severe Acute Respiratory Syndrome - virology ; Severe acute respiratory syndrome-related coronavirus - physiology ; Sialic acids ; Sialic Acids - metabolism ; Swine ; Tissues ; Trachea ; Virology ; Viruses ; Zoonoses
É parte de: PloS one, 2012-04, Vol.7 (4), p.e35876-e35876
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content type line 23
Conceived and designed the experiments: EJS SP. Performed the experiments: HL SB AH SG PP. Analyzed the data: EJS SP. Contributed reagents/materials/analysis tools: JL PSN SB AH SD PP. Wrote the paper: EJS SP.
Descrição: The type II transmembrane serine proteases TMPRSS2 and HAT activate influenza viruses and the SARS-coronavirus (TMPRSS2) in cell culture and may play an important role in viral spread and pathogenesis in the infected host. However, it is at present largely unclear to what extent these proteases are expressed in viral target cells in human tissues. Here, we show that both HAT and TMPRSS2 are coexpressed with 2,6-linked sialic acids, the major receptor determinant of human influenza viruses, throughout the human respiratory tract. Similarly, coexpression of ACE2, the SARS-coronavirus receptor, and TMPRSS2 was frequently found in the upper and lower aerodigestive tract, with the exception of the vocal folds, epiglottis and trachea. Finally, activation of influenza virus was conserved between human, avian and porcine TMPRSS2, suggesting that this protease might activate influenza virus in reservoir-, intermediate- and human hosts. In sum, our results show that TMPRSS2 and HAT are expressed by important influenza and SARS-coronavirus target cells and could thus support viral spread in the human host.
Editor: United States: Public Library of Science
Idioma: Inglês

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Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts

Bertram, Stephanie ; Heurich, Adeline ; Lavender, Hayley ; Gierer, Stefanie ; Danisch, Simon ; Perin, Paula ; Lucas, Jared M ; Nelson, Peter S ; Pöhlmann, Stefan ; Soilleux, Elizabeth J Thiel, Volker

United States: Public Library of Science

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