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Influenza Virus Hemagglutinin HA-2 N-Terminal Fusogenic Peptides Augment Gene Transfer by Transferrin-Polylysine-DNA Complexes: Toward a Synthetic Virus-Like Gene-Transfer Vehicle

Wagner, Ernst ; Plank, Christian ; Zatloukal, Kurt ; Cotten, Matt ; Birnstiel, Max L.

Proceedings of the National Academy of Sciences - PNAS, 1992-09, Vol.89 (17), p.7934-7938 [Periódico revisado por pares]

WASHINGTON: National Academy of Sciences of the United States of America

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Título:
Influenza Virus Hemagglutinin HA-2 N-Terminal Fusogenic Peptides Augment Gene Transfer by Transferrin-Polylysine-DNA Complexes: Toward a Synthetic Virus-Like Gene-Transfer Vehicle
Autor: Wagner, Ernst ; Plank, Christian ; Zatloukal, Kurt ; Cotten, Matt ; Birnstiel, Max L.
Assuntos: Amino Acid Sequence ; Animals ; beta-Galactosidase - genetics ; Cell lines ; Daughter cells ; Deoxyribonucleic acid ; DNA ; Gene Expression ; Genes ; HeLa Cells ; Hemagglutinins, Viral ; Hepatocytes ; Humans ; In Vitro Techniques ; Influenza ; Influenza A virus - chemistry ; influenza virus ; K562 cells ; Liposomes ; Luciferases - genetics ; Mice ; Molecular Sequence Data ; Multidisciplinary Sciences ; Orthomyxoviridae ; Polylysine ; Recombinant Proteins ; Science & Technology ; Science & Technology - Other Topics ; Transfection ; Transferrin ; Tumor Cells, Cultured ; Viral Fusion Proteins ; Viruses
É parte de: Proceedings of the National Academy of Sciences - PNAS, 1992-09, Vol.89 (17), p.7934-7938
Notas: ObjectType-Article-2
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Descrição: Complexes containing plasmid DNA, transferrin-polylysine conjugates, and polylysine-conjugated peptides derived from the N-terminal sequence of the influenza virus hemagglutinin subunit HA-2 have been used for the transfer of luciferase or β-galactosidase marker genes to K562 cells, HeLa cells, and BNL CL.2 hepatocytes. These DNA complexes mimic the entry of viruses into cells, as they contain functions for (i) the packaging of the nucleic acid with polylysine, (ii) the attachment to the cell and receptor-mediated endocytosis with transferrin as a ligand, and (iii) the release from endosomes by using membrane-disrupting influenza peptides. The presence of these influenza peptide conjugates in the DNA complexes renders the complexes active in membrane disruption in a liposome leakage assay and results in a substantial augmentation of the transferrin-polylysine-mediated gene transfer.
Editor: WASHINGTON: National Academy of Sciences of the United States of America
Idioma: Inglês

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Influenza Virus Hemagglutinin HA-2 N-Terminal Fusogenic Peptides Augment Gene Transfer by Transferrin-Polylysine-DNA Complexes: Toward a Synthetic Virus-Like Gene-Transfer Vehicle

Wagner, Ernst ; Plank, Christian ; Zatloukal, Kurt ; Cotten, Matt ; Birnstiel, Max L.

WASHINGTON: National Academy of Sciences of the United States of America

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